Snake venom cardiotoxin induces G-actin polymerization

• Published in: Biochimica et biophysica acta Vol. 966; no. 2; pp. 266 - 268
• Main Authors: Chen, Yee-Hsiung, Chu, Sin-Tak
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 11.08.1988; Elsevier; North-Holland
• Subjects: Actin polymerization; Actins - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Biopolymers - metabolism; Cardiotoxin; Cobra Cardiotoxin Proteins - pharmacology; Contractile proteins; Elapid Venoms - pharmacology; Fundamental and applied biological sciences. Psychology; Holoproteins; In Vitro Techniques; Muscles - drug effects; Muscles - metabolism; Proteins; Rabbit skeletal muscle; Rabbits; Snake venom; Cardiotoxin; Snake venom; Actin polymerization; Rabbit skeletal muscle; Vertebrata; Mammalia; Basic protein; Venom; Actins; Rabbit; Polymerization; Molecular interaction; Reptilia; Lagomorpha; Striated muscle

Snake venom cardiotoxin showed the ability to induce polymerization of G-actin from rabbit skeletal muscle in a low ionic strength buffer composed of 0.2 mM CaCl 2/0.2 mM ATP/0.5 mM mercaptoethanol/2.0 mM Tris-HCl, pH 8.0. The activity was enhanced greatly when 0.4 mM MgCl 2 was present in the buffe...