Measurement of free choline concentrations in maternal and neonatal blood by micropyrolysis gas chromatography

• Published in: Clinica chimica acta Vol. 149; no. 1; pp. 1 - 12
• Main Authors: McMahon, Kathleen E., Farrell, Philip M.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 30.06.1985; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Choline; Choline - blood; Chromatography, Gas - instrumentation; Chromatography, Gas - methods; Erythrocytes - analysis; Female; Fetal Blood - analysis; Fundamental and applied biological sciences. Psychology; Humans; Infant, Newborn; Newborn; Non peptidic neurotransmitters, polyamines; Nutrition; Other biological molecules; Plasma - analysis; Pregnancy; Prematurity; Reference Standards; Respiratory Distress Syndrome, Newborn - blood; Pregnancy; Newborn; Nutrition; Prematurity; Choline; Assay; Human; Gas chromatography; Pyrolysis; Mother; New born; Chromatography; Blood

The development of a micropyrolysis gas Chromatographie technique for the determination of free choline in plasma and erythrocytes is reported as a means to clinically assess choline status. A micropyrolyzer syringe unit was fabricated and the method was standardized through repeated trials with a s...