ORG-2058 as a ligand in the assay of progesterone receptor in breast cancer
Tritiated [(16α-ethyl-21-hydroxy-19-nor-pregn-4-ene-3, 20-dione)-6,7- 3H] (ORG-2058) and 17,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione (R5020) were compared as ligands in the assay of progesterone receptor in human of and rat breast tumors. We found that ORG-2058 is a better ligand because its l...
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Published in | Steroids Vol. 48; no. 5; pp. 419 - 426 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Elsevier Inc
01.11.1986
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Tritiated [(16α-ethyl-21-hydroxy-19-nor-pregn-4-ene-3, 20-dione)-6,7-
3H] (ORG-2058) and 17,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione (R5020) were compared as ligands in the assay of progesterone receptor in human of and rat breast tumors. We found that ORG-2058 is a better ligand because its low nonspecific binding. Most of the nonspecific binding of the other ligand R5020, is to proteins which bind corticosteroids. In cancerous tissue ORG-2058 binds to progesterone receptor linearly in a range of protein concentrations which are normally used in the receptor assay. On the other hand, R5020 exhibits binding linearity over a narrower protein concentration in many tumor biopsies, which may cause severe limitation in the assay procedure or frequent underestimation of receptor content. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0039-128X 1878-5867 |
DOI: | 10.1016/0039-128X(86)90028-0 |