Nicking enzyme-assisted signal-amplifiable Hg2+ detection using upconversion nanoparticles

• Published in: Analytica chimica acta Vol. 1072; pp. 75 - 80
• Main Authors: Vijayan, Anjaly N., Liu, Zhiming, Zhao, Haohan, Zhang, Peng
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.09.2019; Elsevier BV
• Subjects: Amplification; Deoxyribonucleic acid; DNA; Drinking water; Energy transfer; Environmental protection; Enzymes; Fluorides; Luminescence resonance energy transfer (LRET); Mercuric ion; Mercury; Mercury (metal); Nanoparticles; Nicking endonuclease; Nicking enzyme; Selectivity; Signal amplification; Signal monitoring; Single-stranded DNA; Sodium compounds; Thulium; Thymine; Upconversion; Upconversion nanoparticles (UCNPs); Ytterbium; Upconversion nanoparticles (UCNPs); Nicking enzyme; Luminescence resonance energy transfer (LRET); Mercuric ion; Signal amplification

A highly specific and sensitive isothermal method for mercury detection using DNA-conjugated upconversion nanoparticles is reported. A single-stranded DNA containing thymine bases, used as the Hg2+-capturing element through the formation of thymine-Hg2+-thymine complex, is covalently attached to the...