Purification and properties of dyneins from Paramecium cilia

• Published in: Biochimica et biophysica acta Vol. 966; no. 1; pp. 73 - 83
• Main Authors: Travis, Sue M., Nelson, David L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 14.07.1988; Elsevier; North-Holland
• Subjects: Adenosine Triphosphatases - isolation & purification; Adenosine Triphosphate - metabolism; Analytical, structural and metabolic biochemistry; Animals; Antigen-Antibody Reactions; ATPase; Axoneme; Biological and medical sciences; Centrifugation, Density Gradient; Chromatography, Ion Exchange; Cilia - analysis; Cilium; Dynein; Dyneins - immunology; Dyneins - isolation & purification; Dyneins - metabolism; Electrophoresis, Polyacrylamide Gel; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Hydrolases; Ions; Metals - metabolism; Molecular Weight; Paramecium; Paramecium - analysis; Mops; Cilium; ATPase; EHNA; Dynein; Paramecium; Axoneme; Protozoa; Molecular form; Purification; Gel electrophoresis; Enzyme; Ion exchange chromatography; Density gradient centrifugation; Sucrose gradient; Immunoblotting assay; Ciliata; Mg-ATPase

Dynein ATPases purified from Paramecium cilia by salt extraction followed by sucrose density gradient centrifugation and anion exchange chromatography. The two major dyneins sedimented in sucrose gradients as species of 22 S and 12 S. After purification by anion exchange chromatography, their specif...