Studies on the regulation and function of the Klebsiella pneumoniae ntrA gene
The ntrA gene from Klebsiella pneumoniae has been cloned and the product identified as a 76-kDal acidic polypeptide. An ntrA : : lacZ fusion was used to demonstrate that expression of ntrA is not controlled by the nitrogen regulation ( ntr) system and is independent of the nitrogen status of the cel...
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Published in | Gene Vol. 35; no. 3; pp. 297 - 303 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
1985
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | The
ntrA gene from
Klebsiella pneumoniae has been cloned and the product identified as a 76-kDal acidic polypeptide. An
ntrA : :
lacZ fusion was used to demonstrate that expression of
ntrA is not controlled by the nitrogen regulation (
ntr) system and is independent of the nitrogen status of the cell. Studies with multicopy plasmids carrying
ntrA and
rpoD suggest that the
ntrA product competes with the
rpoD product (σ
70 of RNA polymerase) in mediating transcription initiation by RNA polymerase at
ntrA -dependent promoters. No significant homology between
ntrA and
rpoD was detected by Southern blotting. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(85)90008-3 |