Protection against 7,12-dimethylbenzanthracene-induced tumour initiation by protein A in mouse skin

• Published in: Cancer letters Vol. 61; no. 2; pp. 105 - 110
• Main Authors: Kumar, Sushil, Shukla, Yogeshwer, Prasad, Arun Kumar, Verma, A.S., Dwivedi, P.D., Mehrotra, N.K., Ray, P.K.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 10.01.1992; Elsevier
• Subjects: 7,12-dimethylbenzanthracene; 9,10-Dimethyl-1,2-benzanthracene; Animal tumors. Experimental tumors; Animals; Antineoplastic Agents; Biological and medical sciences; Experimental skin tumors; Female; Medical sciences; Mice; mouse skin; protein A; Skin Neoplasms - chemically induced; Skin Neoplasms - prevention & control; Staphylococcal Protein A - administration & dosage; Survival Analysis; Tumors; tumour initiation; tumour initiation; mouse skin; protein A; 7,12-dimethylbenzanthracene; Skin disease; Intraperitoneal administration; Rodentia; Tumor initiation; Glycoproteins; Anticarcinogen; Multiple dose; Prevention; Vertebrata; Experimental disease; Mammalia; Mouse; Animal; Bacteria; Micrococcales; Tumor; Micrococcaceae; Staphylococcus aureus
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/0304-3835(92)90167-T

Protein A is an immunostimulating glycoprotein obtained from Staphylococcus aureus Cowan I. Its antitumour activity is proven in various tumour models. Its ability to provide protection against tumour initiation by the chemical carcinogen 7,12-dimethylbenzanthracene (DMBA) has been investigated in the present study using a mouse skin model of two-stage carcinogenesis. Protein A was administered intraperitoneally (l μg/animal 20 g body wt.) twice a week for 2 weeks, prior to initiation by DMBA. The promotion was performed by twice weekly applications of 12-O-tetradecanoyl phorbol-13-acetate (TPA) (3 or 5 μg/animal in 100 μl acetone). Protein A provided significant protection animals from DMBA-induced tumour initiation as was observed by the decrease in cumulative number of tumours, percent of animals developing tumours, number of tumours per animal and rate of tumour growth. Our data indicate that protein A has anticarcinogenic properties.