Expression of organic osmolyte transporters in cultured rat astrocytes and rat and human cerebral cortex

• Published in: Archives of biochemistry and biophysics Vol. 560; pp. 59 - 72
• Main Authors: Oenarto, Jessica, Görg, Boris, Moos, Michael, Bidmon, Hans-Jürgen, Häussinger, Dieter
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.10.2014
• Subjects: Acetates - toxicity; Ammonia; ammonium acetate; ammonium chloride; animal models; Animals; Astrocytes; Astrocytes - cytology; Astrocytes - drug effects; Astrocytes - metabolism; Cells, Cultured; cerebral cortex; Cerebral Cortex - cytology; Cerebral Cortex - drug effects; Cerebral Cortex - metabolism; diazepam; encephalopathy; gamma-aminobutyric acid; gene expression; Gene Expression Regulation - drug effects; glutamate-ammonia ligase; Hepatic encephalopathy; Humans; Ligation; Male; Membrane Transport Proteins - genetics; messenger RNA; myo-inositol; NAD(P)H oxidase (H2O2-forming); NADP (coenzyme); neurons; Osmolyte transporter; portal vein; Portal Vein - surgery; Rats; taurine; transporters; tumor necrosis factor-alpha; Ammonia; Astrocytes; Hepatic encephalopathy; Osmolyte transporter

•Osmolyte transporter expression in brain is cell-type specific.•HE-relevant factors differently affect osmolyte transporter expression in astrocytes.•Ammonia downregulates SMIT mRNA in a methionine-sulfoximine and oxidative stress dependend way. This study characterizes the expression of the osmoly...