Peptides eluted from HLA-B27 of human splenocytes and blood cells reveal a similar but partially different profile compared to in vitro grown cell lines

• Published in: Immunology letters Vol. 94; no. 3; pp. 261 - 265
• Main Authors: Stodůlková, Eva, Man, Petr, Pohl, Jan, Van Nguyen, Dung, Vaingátová, Silvie, Ivašková, Eva, Pla, Marika, Čapková, Jana, Sedláčková, Marie, Ivanyi, Pavol, Flieger, Miroslav
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.07.2004
• Subjects: Cells, Cultured; HLA-B ∗2705; HLA-B27 Antigen - chemistry; HLA-B27 Antigen - immunology; Humans; LC–MS/MS analysis; Lymphocytes - immunology; Peptide Fragments - chemistry; Peptide Fragments - immunology; Peptide profile; Peptide sequence; Spleen - immunology; BC; SpA; HLA-B ∗2705; LC–MS/MS analysis; Peptide sequence; MHC; HLA; Peptide profile

The sequences and profiles of peptides which bind to HLA-B ∗2705 splenocytes and peripheral blood cells were compared with those previously published from in vitro long-term cell cultures. B ∗2705 peptide profile analysed by solid-phase Edman degradation and 15 individual peptide sequences determine...