Creation of knock out and knock in mice by CRISPR/Cas9 to validate candidate genes for human male infertility, interest, difficulties and feasibility

• Published in: Molecular and cellular endocrinology Vol. 468; pp. 70 - 80
• Main Authors: Kherraf, Zine-Eddine, Conne, Beatrice, Amiri-Yekta, Amir, Kent, Marie Christou, Coutton, Charles, Escoffier, Jessica, Nef, Serge, Arnoult, Christophe, Ray, Pierre F.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 15.06.2018
• Subjects: animal models; Animals; cell division; CRISPR-Associated Protein 9 - metabolism; CRISPR-Cas systems; CRISPR-Cas Systems - genetics; CRISPR/Cas9; Female; flagellum; gene editing; Gene Knock-In Techniques - methods; genes; Genetic Association Studies; genetic variation; High throughput sequencing; high-throughput nucleotide sequencing; homozygosity; Humans; Infertility, Male - genetics; Knock out mice; loci; Male; male sterility; men; mice; Mice, Inbred C57BL; Mice, Knockout; mutation; Mutation - genetics; Nucleotide Motifs - genetics; pathophysiology; phenotype; pups; somatic cells; Sperm flagella; Spermatogenesis; spermatozoa; tail; Sperm flagella; High throughput sequencing; CRISPR/Cas9; Spermatogenesis; Knock out mice

High throughput sequencing (HTS) and CRISPR/Cas9 are two recent technologies that are currently revolutionizing biological and clinical research. Both techniques are complementary as HTS permits to identify new genetic variants and genes involved in various pathologies and CRISPR/Cas9 permits to cre...