Creation of knock out and knock in mice by CRISPR/Cas9 to validate candidate genes for human male infertility, interest, difficulties and feasibility - Kherraf, Zine-Eddine; Conne, Beatrice; Amiri-Yekta, Amir; Kent, Marie Christou; Coutton, Charles; Escoffier, Jessica; Nef, Serge; Arnoult, Christophe; Ray, Pierre F. (Molecular and cellular endocrinology, 2018)
Keywords: animal models; Animals; cell division; CRISPR-Associated Protein 9 - metabolism; CRISPR-Cas systems; CRISPR-Cas Systems - genetics; CRISPR/Cas9; Female; flagellum; gene editing; Gene Knock-In Techniques - methods; genes; Genetic Association Studies; genetic variation; High throughput sequencing; high-throughput nucleotide sequencing; homozygosity; Humans; Infertility, Male - genetics; Knock out mice; loci; Male; male sterility; men; mice; Mice, Inbred C57BL; Mice, Knockout; mutation; Mutation - genetics; Nucleotide Motifs - genetics; pathophysiology; phenotype; pups; somatic cells; Sperm flagella; Spermatogenesis; spermatozoa; tail; Sperm flagella; High throughput sequencing; CRISPR/Cas9; Spermatogenesis; Knock out mice