A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for the diagnosis of marker proteins

A single-step, easy-to-use enzyme immunoassay capillary sensor, composed of functional multilayer coatings, was developed in this study. The coatings were composed of substrate-immobilized hydrophobic coating, hydrogel coating, and soluble coating containing an enzyme-labeled antibody. The response...

Full description

Saved in:
Bibliographic Details
Published inAnalyst (London) Vol. 140; no. 5; pp. 1459 - 1465
Main Authors Funano, Shun-ichi, Sugahara, Masato, Henares, Terence G, Sueyoshi, Kenji, Endo, Tatsuro, Hisamoto, Hideaki
Format Journal Article
LanguageEnglish
Published England 07.03.2015
Subjects
alpha-Fetoproteins - analysis
Antibodies
Antigen-Antibody Complex
Antigens
Biomarkers - analysis
C-Reactive Protein - analysis
Capillarity
Coatings
Electrophoresis, Capillary - methods
Enzymes
Glycated Hemoglobin A - analysis
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Hydrogels
Immunoenzyme Techniques - methods
Markers
Proteins
Sensors
Online AccessGet full text

Cover

Abstract A single-step, easy-to-use enzyme immunoassay capillary sensor, composed of functional multilayer coatings, was developed in this study. The coatings were composed of substrate-immobilized hydrophobic coating, hydrogel coating, and soluble coating containing an enzyme-labeled antibody. The response mechanism involved a spontaneous immunoreaction triggered by capillary action-mediated introduction of a sample antigen solution and subsequent separation of unreacted enzyme-labeled antibodies and antigen-enzyme-labeled antibody complexes by the molecular sieving effect of the hydrogel. An enzyme reaction at the substrate-immobilized hydrophobic coating/hydrogel coating interface resulted in a protein-selective fluorescence response. An antigen concentration-dependent response was obtained for diagnostic marker protein samples (hemoglobin A1c (HbA1c), 7.14-16.7 mg mL(-1); alpha-fetoprotein (AFP), 1.4-140 ng mL(-1); C-reactive protein (CRP), 0.5-10 μg mL(-1)) that cover a clinically important concentration range. The successful measurement of CRP in diluted serum samples demonstrated the application of this capillary sensor.
AbstractList A single-step, easy-to-use enzyme immunoassay capillary sensor, composed of functional multilayer coatings, was developed in this study. The coatings were composed of substrate-immobilized hydrophobic coating, hydrogel coating, and soluble coating containing an enzyme-labeled antibody. The response mechanism involved a spontaneous immunoreaction triggered by capillary action-mediated introduction of a sample antigen solution and subsequent separation of unreacted enzyme-labeled antibodies and antigen-enzyme-labeled antibody complexes by the molecular sieving effect of the hydrogel. An enzyme reaction at the substrate-immobilized hydrophobic coating/hydrogel coating interface resulted in a protein-selective fluorescence response. An antigen concentration-dependent response was obtained for diagnostic marker protein samples (hemoglobin A1c (HbA1c), 7.14-16.7 mg mL(-1); alpha-fetoprotein (AFP), 1.4-140 ng mL(-1); C-reactive protein (CRP), 0.5-10 μg mL(-1)) that cover a clinically important concentration range. The successful measurement of CRP in diluted serum samples demonstrated the application of this capillary sensor.
A single-step, easy-to-use enzyme immunoassay capillary sensor, composed of functional multilayer coatings, was developed in this study. The coatings were composed of substrate-immobilized hydrophobic coating, hydrogel coating, and soluble coating containing an enzyme-labeled antibody. The response mechanism involved a spontaneous immunoreaction triggered by capillary action-mediated introduction of a sample antigen solution and subsequent separation of unreacted enzyme-labeled antibodies and antigen-enzyme-labeled antibody complexes by the molecular sieving effect of the hydrogel. An enzyme reaction at the substrate-immobilized hydrophobic coating/hydrogel coating interface resulted in a protein-selective fluorescence response. An antigen concentration-dependent response was obtained for diagnostic marker protein samples (hemoglobin A1c (HbA1c), 7.14–16.7 mg mL −1 ; alpha-fetoprotein (AFP), 1.4–140 ng mL −1 ; C-reactive protein (CRP), 0.5–10 μg mL −1 ) that cover a clinically important concentration range. The successful measurement of CRP in diluted serum samples demonstrated the application of this capillary sensor.
A single-step, easy-to-use enzyme immunoassay capillary sensor, composed of functional multilayer coatings, was developed in this study. The coatings were composed of substrate-immobilized hydrophobic coating, hydrogel coating, and soluble coating containing an enzyme-labeled antibody. The response mechanism involved a spontaneous immunoreaction triggered by capillary action-mediated introduction of a sample antigen solution and subsequent separation of unreacted enzyme-labeled antibodies and antigen-enzyme-labeled antibody complexes by the molecular sieving effect of the hydrogel. An enzyme reaction at the substrate-immobilized hydrophobic coating/hydrogel coating interface resulted in a protein-selective fluorescence response. An antigen concentration-dependent response was obtained for diagnostic marker protein samples (hemoglobin A1c (HbA1c), 7.14-16.7 mg mL super(-1); alpha-fetoprotein (AFP), 1.4-140 ng mL super(-1); C-reactive protein (CRP), 0.5-10 mu g mL super(-1)) that cover a clinically important concentration range. The successful measurement of CRP in diluted serum samples demonstrated the application of this capillary sensor.
Author Endo, Tatsuro
Funano, Shun-ichi
Hisamoto, Hideaki
Sueyoshi, Kenji
Sugahara, Masato
Henares, Terence G
Author_xml – sequence: 1
  givenname: Shun-ichi
  surname: Funano
  fullname: Funano, Shun-ichi
  email: sueyoshi@chem.osakafu-u.ac.jp
  organization: Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai City, Osaka 599-8531, Japan. sueyoshi@chem.osakafu-u.ac.jp
– sequence: 2
  givenname: Masato
  surname: Sugahara
  fullname: Sugahara, Masato
– sequence: 3
  givenname: Terence G
  surname: Henares
  fullname: Henares, Terence G
– sequence: 4
  givenname: Kenji
  surname: Sueyoshi
  fullname: Sueyoshi, Kenji
– sequence: 5
  givenname: Tatsuro
  surname: Endo
  fullname: Endo, Tatsuro
– sequence: 6
  givenname: Hideaki
  surname: Hisamoto
  fullname: Hisamoto, Hideaki
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25599100$$D View this record in MEDLINE/PubMed
BookMark eNqFkT1v3DAMhoUiQXNJuvQHBBqLAm4kS7Kt8XBI0wJBs2Q3eDKVqrEl17QHZ-s_ry5fayaCwMOHIN9TdhRTRMY-S_FNCmUvnYYoZN1I-MA2UlW6MKZsjthGCKGKsjL6hJ0S_cmtFEZ8ZCelMdZKITbs35ZTiPc9FjTjyDE-rgPyMAxLTEAEK3cwhr6HaeWEkdLEXRrGRNjx5LlfoptDitDzYenn0MOKBwLmLCXuMz7_Rt4FuI-JAh1mBpgeMjROacYQ6Zwde-gJP73UM3b3_epu96O4ub3-udveFE411Vw0pfEgSoO6wcbVncSucgqt9503-877PVSiViXYvRFGOwfaOlN3TalLBKvO2Jdnbd77d0Ga2yGQw3xZxLRQK6vKNrbS2fE-akyd32pVRr8-o25KRBP6dpxCPnBtpWgP4bQ7vf31FM42wxcv3mU_YPeGvqah_gPSJ49f
CitedBy_id crossref_primary_10_1016_j_snb_2016_04_072
crossref_primary_10_2116_analsci_18C008
crossref_primary_10_1021_acs_analchem_0c05007
crossref_primary_10_1016_j_bios_2018_11_013
crossref_primary_10_1016_j_snb_2015_05_001
crossref_primary_10_2116_analsci_31_1155
crossref_primary_10_1039_C5AN02637G
crossref_primary_10_1371_journal_pone_0194631
crossref_primary_10_1016_j_talanta_2020_121725
crossref_primary_10_1016_j_snb_2021_130711
crossref_primary_10_1039_D2AN00115B
crossref_primary_10_3390_s150717366
crossref_primary_10_1021_acsanm_1c00672
crossref_primary_10_1016_j_snb_2017_11_183
crossref_primary_10_1039_D1AN00447F
crossref_primary_10_1109_JSEN_2020_3028610
crossref_primary_10_2116_analsci_19C013
crossref_primary_10_3390_s18010055
crossref_primary_10_1016_j_flatc_2018_12_003
crossref_primary_10_1039_D0AN00335B
crossref_primary_10_15583_jpchrom_2015_035
crossref_primary_10_1039_C6AN02485H
crossref_primary_10_1039_C7AN00523G
Cites_doi 10.1016/j.aca.2008.11.019
10.1039/C3RA46976J
10.1097/EDE.0b013e3181e9e978
10.1016/j.talanta.2009.12.058
10.1039/b818430e
10.1007/s00216-010-4011-2
10.1016/j.jpba.2013.07.008
10.1039/c3an00098b
10.1620/tjem.220.199
10.1002/elps.201100218
10.1002/elan.201200525
10.1046/j.1365-2036.2003.01577.x
10.1007/s00604-010-0333-1
10.1016/j.electacta.2012.07.074
10.1246/cl.2010.436
10.1021/ac061245i
10.1007/s10404-010-0718-9
10.1007/s12161-014-9797-7
10.1021/ac035385t
10.1021/ac9013989
10.1021/ac402055n
10.1016/j.bios.2011.07.074
10.1007/s10404-012-0980-0
10.1039/c2lc40994a
10.1039/b815475a
10.1021/ac000991z
10.1039/c2lc21242k
10.1021/ac3031543
10.2116/analsci.30.7
10.1016/j.jviromet.2009.04.003
10.1016/j.trac.2011.06.020
10.1039/c3an36785a
10.1039/c3lc50775k
10.1038/jid.2013.287
10.1007/s00216-006-0549-4
10.1021/am5012782
10.1016/j.snb.2010.06.035
10.4103/0022-3859.101392
10.1039/c2ra21843g
10.1007/s00216-010-3678-8
10.1016/j.ab.2013.05.021
10.1021/es026191i
10.1039/C1LC20651F
10.1016/0022-1759(92)90061-W
10.1021/ac015645b
10.1016/j.aca.2007.02.060
10.1016/j.aca.2005.05.028
10.1039/c3lc50169h
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
7SR
7U5
8BQ
8FD
JG9
L7M
DOI 10.1039/c4an01781a
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
METADEX
Technology Research Database
Materials Research Database
Advanced Technologies Database with Aerospace
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
Materials Research Database
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
Technology Research Database
Advanced Technologies Database with Aerospace
METADEX
DatabaseTitleList MEDLINE
MEDLINE - Academic
CrossRef
Materials Research Database
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1364-5528
EndPage 1465
ExternalDocumentID 10_1039_C4AN01781A
25599100
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
-JG
-~X
.HR
0-7
0R~
23M
2WC
4.4
53G
5RE
705
70~
7~J
AAEMU
AAIWI
AANOJ
AAXHV
AAXPP
ABASK
ABDVN
ABGFH
ABJNI
ABOCM
ABPDG
ABRYZ
ACGFS
ACIWK
ACLDK
ADMRA
AENEX
AETIL
AFOGI
AFVBQ
AGEGJ
AGRSR
AGSTE
ALMA_UNASSIGNED_HOLDINGS
ANUXI
ASKNT
AUDPV
AZFZN
BLAPV
BSQNT
C6K
CGR
COF
CS3
CUY
CVF
EBS
ECGLT
ECM
EE0
EF-
EIF
EJD
F5P
GNO
HZ~
H~N
IDZ
J3G
J3H
J3I
M4U
N9A
NPM
O9-
P2P
R7B
R7E
RAOCF
RCNCU
RIG
ROL
RPMJG
RRA
RRC
RSCEA
SKM
SKR
SKZ
SLC
SLF
TN5
UPT
VH6
WH7
XFK
~02
.GJ
0UZ
186
1TJ
3EH
3O-
71~
AAJAE
AAMEH
AAWGC
AAYXX
ABEMK
ABXOH
ACHDF
ACMRT
ADSRN
AEFDR
AENGV
AESAV
AFFNX
AFLYV
AGKEF
AHGCF
AHGXI
AIDUJ
ANBJS
ANLMG
APEMP
ASPBG
AVWKF
BBWZM
C1A
CAG
CITATION
EEHRC
GGIMP
H13
IDY
KC5
L-8
LPU
MVM
NDZJH
R56
RCLXC
RNS
RRXOS
SC5
SLH
XOL
XXG
ZCG
ZKB
ZXP
7X8
7SR
7U5
8BQ
8FD
JG9
L7M
ID FETCH-LOGICAL-c386t-825fa025e48e8c7d1ed6c3e9ffdf5bdffba60732a9b5054cca49c57d8242ea93
ISSN 0003-2654
IngestDate Thu Jul 25 10:10:02 EDT 2024
Fri Aug 16 14:21:20 EDT 2024
Fri Aug 23 00:42:33 EDT 2024
Thu May 23 23:21:10 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 5
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c386t-825fa025e48e8c7d1ed6c3e9ffdf5bdffba60732a9b5054cca49c57d8242ea93
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 25599100
PQID 1655726593
PQPubID 23479
PageCount 7
ParticipantIDs proquest_miscellaneous_1669896407
proquest_miscellaneous_1655726593
crossref_primary_10_1039_C4AN01781A
pubmed_primary_25599100
PublicationCentury 2000
PublicationDate 2015-03-07
PublicationDateYYYYMMDD 2015-03-07
PublicationDate_xml – month: 03
  year: 2015
  text: 2015-03-07
  day: 07
PublicationDecade 2010
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle Analyst (London)
PublicationTitleAlternate Analyst
PublicationYear 2015
References Hisamoto (C4AN01781A-(cit48)/*[position()=1]) 2006; 556
Kovarik (C4AN01781A-(cit11)/*[position()=1]) 2013; 85
Mirasoli (C4AN01781A-(cit12)/*[position()=1]) 2014; 87
Martinez (C4AN01781A-(cit32)/*[position()=1]) 2010; 82
Yetisen (C4AN01781A-(cit33)/*[position()=1]) 2013; 13
Barnighausen (C4AN01781A-(cit3)/*[position()=1]) 2010; 21
Cho (C4AN01781A-(cit25)/*[position()=1]) 2009; 632
Yakovleva (C4AN01781A-(cit23)/*[position()=1]) 2002; 74
Xu (C4AN01781A-(cit7)/*[position()=1]) 2014; 7
Ohashi (C4AN01781A-(cit15)/*[position()=1]) 2009; 9
Wakayama (C4AN01781A-(cit47)/*[position()=1]) 2013; 13
Yang (C4AN01781A-(cit50)/*[position()=1]) 1980; 87
Henares (C4AN01781A-(cit38)/*[position()=1]) 2010; 149
Sasso (C4AN01781A-(cit20)/*[position()=1]) 2012; 13
Zhang (C4AN01781A-(cit24)/*[position()=1]) 2011; 29
Uchiyama (C4AN01781A-(cit46)/*[position()=1]) 2012; 12
Gan (C4AN01781A-(cit1)/*[position()=1]) 2013; 133
Henares (C4AN01781A-(cit36)/*[position()=1]) 2014; 30
Tsutsumi (C4AN01781A-(cit49)/*[position()=1]) 2010; 39
Workman (C4AN01781A-(cit29)/*[position()=1]) 2009; 160
Abe (C4AN01781A-(cit30)/*[position()=1]) 2010; 398
Ishimoto (C4AN01781A-(cit45)/*[position()=1]) 2014; 4
Hisamoto (C4AN01781A-(cit40)/*[position()=1]) 2004; 76
C4AN01781A-(cit53)/*[position()=1]
Kiba (C4AN01781A-(cit26)/*[position()=1]) 2012; 81
Hervas (C4AN01781A-(cit9)/*[position()=1]) 2012; 31
Ishimoto (C4AN01781A-(cit44)/*[position()=1]) 2013; 138
Henares (C4AN01781A-(cit42)/*[position()=1]) 2007; 79
Huang (C4AN01781A-(cit16)/*[position()=1]) 2011; 32
Kabir (C4AN01781A-(cit5)/*[position()=1]) 2003; 17
Sato (C4AN01781A-(cit22)/*[position()=1]) 2001; 73
Henares (C4AN01781A-(cit39)/*[position()=1]) 2013; 138
Tanaka (C4AN01781A-(cit6)/*[position()=1]) 2010; 220
Henares (C4AN01781A-(cit37)/*[position()=1]) 2007; 589
Tanaka (C4AN01781A-(cit35)/*[position()=1]) 2006; 385
Funano (C4AN01781A-(cit14)/*[position()=1]) 2013; 440
Pauling (C4AN01781A-(cit51)/*[position()=1]) 1952; 96
Fujii (C4AN01781A-(cit41)/*[position()=1]) 2012; 12
Lee (C4AN01781A-(cit18)/*[position()=1]) 2010; 81
Oyama (C4AN01781A-(cit34)/*[position()=1]) 2012; 12
Kong (C4AN01781A-(cit17)/*[position()=1]) 2009; 9
Smith (C4AN01781A-(cit52)/*[position()=1]) 1980; 605
C4AN01781A-(cit54)/*[position()=1]
Hartwell (C4AN01781A-(cit8)/*[position()=1]) 2010; 169
Jiang (C4AN01781A-(cit10)/*[position()=1]) 2011; 10
Liu (C4AN01781A-(cit21)/*[position()=1]) 2013; 25
Porstmann (C4AN01781A-(cit2)/*[position()=1]) 1992; 150
Ng (C4AN01781A-(cit13)/*[position()=1]) 2010; 397
Li (C4AN01781A-(cit28)/*[position()=1]) 2014; 6
Kimura (C4AN01781A-(cit43)/*[position()=1]) 2012; 2
Garimella (C4AN01781A-(cit4)/*[position()=1]) 2012; 58
Ohashi (C4AN01781A-(cit19)/*[position()=1]) 2010
Kim (C4AN01781A-(cit27)/*[position()=1]) 2003; 37
Liu (C4AN01781A-(cit31)/*[position()=1]) 2013; 85
References_xml – volume: 632
  start-page: 247
  year: 2009
  ident: C4AN01781A-(cit25)/*[position()=1]
  publication-title: Anal. Chim. Acta
  doi: 10.1016/j.aca.2008.11.019
  contributor:
    fullname: Cho
– volume: 4
  start-page: 7682
  year: 2014
  ident: C4AN01781A-(cit45)/*[position()=1]
  publication-title: RSC Adv.
  doi: 10.1039/C3RA46976J
  contributor:
    fullname: Ishimoto
– volume: 21
  start-page: 685
  year: 2010
  ident: C4AN01781A-(cit3)/*[position()=1]
  publication-title: Epidemiology
  doi: 10.1097/EDE.0b013e3181e9e978
  contributor:
    fullname: Barnighausen
– volume: 81
  start-page: 657
  year: 2010
  ident: C4AN01781A-(cit18)/*[position()=1]
  publication-title: Talanta
  doi: 10.1016/j.talanta.2009.12.058
  contributor:
    fullname: Lee
– volume: 9
  start-page: 1541
  year: 2009
  ident: C4AN01781A-(cit17)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/b818430e
  contributor:
    fullname: Kong
– volume: 398
  start-page: 885
  year: 2010
  ident: C4AN01781A-(cit30)/*[position()=1]
  publication-title: Anal. Bioanal. Chem.
  doi: 10.1007/s00216-010-4011-2
  contributor:
    fullname: Abe
– volume: 87
  start-page: 1003
  year: 1980
  ident: C4AN01781A-(cit50)/*[position()=1]
  publication-title: J. Biochem.
  contributor:
    fullname: Yang
– volume: 87
  start-page: 36
  year: 2014
  ident: C4AN01781A-(cit12)/*[position()=1]
  publication-title: J. Pharm. Biomed. Anal.
  doi: 10.1016/j.jpba.2013.07.008
  contributor:
    fullname: Mirasoli
– volume: 138
  start-page: 3139
  year: 2013
  ident: C4AN01781A-(cit39)/*[position()=1]
  publication-title: Analyst
  doi: 10.1039/c3an00098b
  contributor:
    fullname: Henares
– volume: 220
  start-page: 199
  year: 2010
  ident: C4AN01781A-(cit6)/*[position()=1]
  publication-title: Tohoku J. Exp. Med.
  doi: 10.1620/tjem.220.199
  contributor:
    fullname: Tanaka
– volume: 32
  start-page: 3196
  year: 2011
  ident: C4AN01781A-(cit16)/*[position()=1]
  publication-title: Electrophoresis
  doi: 10.1002/elps.201100218
  contributor:
    fullname: Huang
– volume: 25
  start-page: 1050
  year: 2013
  ident: C4AN01781A-(cit21)/*[position()=1]
  publication-title: Electroanalysis
  doi: 10.1002/elan.201200525
  contributor:
    fullname: Liu
– volume: 17
  start-page: 1345
  year: 2003
  ident: C4AN01781A-(cit5)/*[position()=1]
  publication-title: Aliment. Pharmacol. Ther.
  doi: 10.1046/j.1365-2036.2003.01577.x
  contributor:
    fullname: Kabir
– volume: 169
  start-page: 201
  year: 2010
  ident: C4AN01781A-(cit8)/*[position()=1]
  publication-title: Microchim. Acta
  doi: 10.1007/s00604-010-0333-1
  contributor:
    fullname: Hartwell
– volume: 81
  start-page: 14
  year: 2012
  ident: C4AN01781A-(cit26)/*[position()=1]
  publication-title: Electrochim. Acta
  doi: 10.1016/j.electacta.2012.07.074
  contributor:
    fullname: Kiba
– ident: C4AN01781A-(cit54)/*[position()=1]
– volume: 39
  start-page: 436
  year: 2010
  ident: C4AN01781A-(cit49)/*[position()=1]
  publication-title: Chem. Lett.
  doi: 10.1246/cl.2010.436
  contributor:
    fullname: Tsutsumi
– volume: 79
  start-page: 908
  year: 2007
  ident: C4AN01781A-(cit42)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac061245i
  contributor:
    fullname: Henares
– volume: 10
  start-page: 941
  year: 2011
  ident: C4AN01781A-(cit10)/*[position()=1]
  publication-title: Microfluid. Nanofluid.
  doi: 10.1007/s10404-010-0718-9
  contributor:
    fullname: Jiang
– volume: 7
  start-page: 1619
  year: 2014
  ident: C4AN01781A-(cit7)/*[position()=1]
  publication-title: Food Anal. Method.
  doi: 10.1007/s12161-014-9797-7
  contributor:
    fullname: Xu
– volume: 76
  start-page: 3222
  year: 2004
  ident: C4AN01781A-(cit40)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac035385t
  contributor:
    fullname: Hisamoto
– volume: 82
  start-page: 3
  year: 2010
  ident: C4AN01781A-(cit32)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac9013989
  contributor:
    fullname: Martinez
– volume: 85
  start-page: 10270
  year: 2013
  ident: C4AN01781A-(cit31)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac402055n
  contributor:
    fullname: Liu
– volume: 29
  start-page: 89
  year: 2011
  ident: C4AN01781A-(cit24)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2011.07.074
  contributor:
    fullname: Zhang
– volume: 13
  start-page: 603
  year: 2012
  ident: C4AN01781A-(cit20)/*[position()=1]
  publication-title: Microfluid. Nanofluid.
  doi: 10.1007/s10404-012-0980-0
  contributor:
    fullname: Sasso
– volume: 12
  start-page: 5155
  year: 2012
  ident: C4AN01781A-(cit34)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/c2lc40994a
  contributor:
    fullname: Oyama
– volume: 9
  start-page: 991
  year: 2009
  ident: C4AN01781A-(cit15)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/b815475a
  contributor:
    fullname: Ohashi
– volume: 73
  start-page: 1213
  year: 2001
  ident: C4AN01781A-(cit22)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac000991z
  contributor:
    fullname: Sato
– volume: 12
  start-page: 1522
  year: 2012
  ident: C4AN01781A-(cit41)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/c2lc21242k
  contributor:
    fullname: Fujii
– ident: C4AN01781A-(cit53)/*[position()=1]
– volume: 85
  start-page: 451
  year: 2013
  ident: C4AN01781A-(cit11)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac3031543
  contributor:
    fullname: Kovarik
– volume: 30
  start-page: 7
  year: 2014
  ident: C4AN01781A-(cit36)/*[position()=1]
  publication-title: Anal. Sci.
  doi: 10.2116/analsci.30.7
  contributor:
    fullname: Henares
– volume: 160
  start-page: 14
  year: 2009
  ident: C4AN01781A-(cit29)/*[position()=1]
  publication-title: J. Virol. Methods
  doi: 10.1016/j.jviromet.2009.04.003
  contributor:
    fullname: Workman
– volume: 31
  start-page: 109
  year: 2012
  ident: C4AN01781A-(cit9)/*[position()=1]
  publication-title: TrAC, Trends Anal. Chem.
  doi: 10.1016/j.trac.2011.06.020
  contributor:
    fullname: Hervas
– volume: 138
  start-page: 3158
  year: 2013
  ident: C4AN01781A-(cit44)/*[position()=1]
  publication-title: Analyst
  doi: 10.1039/c3an36785a
  contributor:
    fullname: Ishimoto
– volume: 13
  start-page: 4304
  year: 2013
  ident: C4AN01781A-(cit47)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/c3lc50775k
  contributor:
    fullname: Wakayama
– volume: 133
  start-page: E10
  year: 2013
  ident: C4AN01781A-(cit1)/*[position()=1]
  publication-title: J. Invest. Dermatol.
  doi: 10.1038/jid.2013.287
  contributor:
    fullname: Gan
– volume: 385
  start-page: 1414
  year: 2006
  ident: C4AN01781A-(cit35)/*[position()=1]
  publication-title: Anal. Bioanal. Chem.
  doi: 10.1007/s00216-006-0549-4
  contributor:
    fullname: Tanaka
– volume: 6
  start-page: 6406
  year: 2014
  ident: C4AN01781A-(cit28)/*[position()=1]
  publication-title: ACS Appl. Mater. Interfaces
  doi: 10.1021/am5012782
  contributor:
    fullname: Li
– volume: 149
  start-page: 319
  year: 2010
  ident: C4AN01781A-(cit38)/*[position()=1]
  publication-title: Sens. Actuators, B
  doi: 10.1016/j.snb.2010.06.035
  contributor:
    fullname: Henares
– volume: 58
  start-page: 194
  year: 2012
  ident: C4AN01781A-(cit4)/*[position()=1]
  publication-title: J. Postgrad. Med.
  doi: 10.4103/0022-3859.101392
  contributor:
    fullname: Garimella
– volume: 605
  start-page: 1
  year: 1980
  ident: C4AN01781A-(cit52)/*[position()=1]
  publication-title: Biochim. Biophys. Acta
  contributor:
    fullname: Smith
– volume: 2
  start-page: 9525
  year: 2012
  ident: C4AN01781A-(cit43)/*[position()=1]
  publication-title: RSC Adv.
  doi: 10.1039/c2ra21843g
  contributor:
    fullname: Kimura
– volume: 397
  start-page: 991
  year: 2010
  ident: C4AN01781A-(cit13)/*[position()=1]
  publication-title: Anal. Bioanal. Chem.
  doi: 10.1007/s00216-010-3678-8
  contributor:
    fullname: Ng
– start-page: 4
  year: 2010
  ident: C4AN01781A-(cit19)/*[position()=1]
  publication-title: Biomicrofluidics
  contributor:
    fullname: Ohashi
– volume: 440
  start-page: 137
  year: 2013
  ident: C4AN01781A-(cit14)/*[position()=1]
  publication-title: Anal. Biochem.
  doi: 10.1016/j.ab.2013.05.021
  contributor:
    fullname: Funano
– volume: 37
  start-page: 1899
  year: 2003
  ident: C4AN01781A-(cit27)/*[position()=1]
  publication-title: Environ. Sci. Technol.
  doi: 10.1021/es026191i
  contributor:
    fullname: Kim
– volume: 12
  start-page: 204
  year: 2012
  ident: C4AN01781A-(cit46)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/C1LC20651F
  contributor:
    fullname: Uchiyama
– volume: 150
  start-page: 5
  year: 1992
  ident: C4AN01781A-(cit2)/*[position()=1]
  publication-title: J. Immunol. Methods
  doi: 10.1016/0022-1759(92)90061-W
  contributor:
    fullname: Porstmann
– volume: 74
  start-page: 2994
  year: 2002
  ident: C4AN01781A-(cit23)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac015645b
  contributor:
    fullname: Yakovleva
– volume: 589
  start-page: 173
  year: 2007
  ident: C4AN01781A-(cit37)/*[position()=1]
  publication-title: Anal. Chim. Acta
  doi: 10.1016/j.aca.2007.02.060
  contributor:
    fullname: Henares
– volume: 556
  start-page: 164
  year: 2006
  ident: C4AN01781A-(cit48)/*[position()=1]
  publication-title: Anal. Chim. Acta
  doi: 10.1016/j.aca.2005.05.028
  contributor:
    fullname: Hisamoto
– volume: 96
  start-page: 556
  year: 1952
  ident: C4AN01781A-(cit51)/*[position()=1]
  publication-title: Proc. Am. Philos. Soc.
  contributor:
    fullname: Pauling
– volume: 13
  start-page: 2210
  year: 2013
  ident: C4AN01781A-(cit33)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/c3lc50169h
  contributor:
    fullname: Yetisen
SSID ssj0001050
Score 2.3143566
Snippet A single-step, easy-to-use enzyme immunoassay capillary sensor, composed of functional multilayer coatings, was developed in this study. The coatings were...
SourceID proquest
crossref
pubmed
SourceType Aggregation Database
Index Database
StartPage 1459
SubjectTerms alpha-Fetoproteins - analysis
Antibodies
Antigen-Antibody Complex
Antigens
Biomarkers - analysis
C-Reactive Protein - analysis
Capillarity
Coatings
Electrophoresis, Capillary - methods
Enzymes
Glycated Hemoglobin A - analysis
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Hydrogels
Immunoenzyme Techniques - methods
Markers
Proteins
Sensors
Title A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for the diagnosis of marker proteins
URI https://www.ncbi.nlm.nih.gov/pubmed/25599100
https://search.proquest.com/docview/1655726593
https://search.proquest.com/docview/1669896407
Volume 140
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELbK7gEuiDflJSO4RYG87NrHqNplQWgvFGlvkes4NGibVE1y6N648qsZ23ESBIuASxQldiJ5Po0_2zPfIPSaxoLJXMEylcjAT5iUvgDW4BOYukWkeBBJE-V7Ts8-Jx8uyMVs9n0StdS16zfy6rd5Jf9jVXgGdtVZsv9g2eGj8ADuwb5wBQvD9a9snHp6pX-pfDDVzlPV1WGrvFJnfNTAicXBk2KnqwrtD14Dy9V6byLI6wZIJnBEPaX1O4EmrPBSAP2GFqI1pTxd_GFug_GsbslWR_PsPaPuUPYbfYMUs5Y3aac1QoZNhtOuEqbGt_dp01V-KTfleBr1RWjRaJs41Ii2HrdnK50cZRDVi-G-G3upQ91syj616Gs53b0IiQnfsjOssh43polPSJ8h7lyylXDqsUcmDjZMrID4L54_iLVwqkxEBT6GhWLaCKy22xoMGIG1MAjG2W-ISXSvbqDjaMEJLOSP05PV-4_DvA5MNHACtzF_O_5KC0r3nX9mN9csWQx1Wd1Bt_s1B04tgO6imaruoZtLV-rvPvqW4gmQsAUSngAJD0DCFkjYAQnXBR6BhEcgYQckDEDCACQ8AEn3sUDCDkgP0Or0ZLU88_vaHL6MGW19FpFCAF9WCVNMLvJQ5VTGihdFXpB1XhRrQWH2iARfA8dOwE8kXJJFzoASKsHjh-ioqiv1GOE85IJFknMlWQJfYpTLKIAmVABV4mSOXrkhzXZWgSUzkRMxz5ZJem5skM7RSzfaGQyePvUSlaq7JgspIYuIEh7_qY2uo6rPtOfokTXV8C9n2ifXvnmKbo3QfoaO2n2nngNVbdcvegz9AElynJk
link.rule.ids 315,786,790,27957,27958
linkProvider Royal Society of Chemistry
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+single-step+enzyme+immunoassay+capillary+sensor+composed+of+functional+multilayer+coatings+for+the+diagnosis+of+marker+proteins&rft.jtitle=Analyst+%28London%29&rft.au=Funano%2C+Shun-ichi&rft.au=Sugahara%2C+Masato&rft.au=Henares%2C+Terence+G&rft.au=Sueyoshi%2C+Kenji&rft.date=2015-03-07&rft.eissn=1364-5528&rft.volume=140&rft.issue=5&rft.spage=1459&rft_id=info:doi/10.1039%2Fc4an01781a&rft_id=info%3Apmid%2F25599100&rft.externalDocID=25599100
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0003-2654&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0003-2654&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0003-2654&client=summon