Enzyme-linked immunosorbent assay for group A Streptococcal anti-DNase B in human sera, using recombinant proteins - Comparison to the DNA methyl green micromethod

• Published in: Journal of immunological methods Vol. 451; pp. 111 - 117
• Main Authors: Das, Sarita, Dileepan, T., Johnson, D.R., Kaplan, E.L., Patrick Cleary, P.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2017
• Subjects: antibodies; Antibodies, Bacterial - blood; antigens; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Biomarkers - blood; Coloring Agents - chemistry; deoxyribonucleases; Deoxyribonucleases - genetics; Deoxyribonucleases - immunology; DNA; DNA methyl green micromethod; dnaseB; ELISA; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; genes; Group A Streptococcus; His-tagged DNase B; Humans; Methyl Green - chemistry; nationalities and ethnic groups; neutralization; Polymerase Chain Reaction; Predictive Value of Tests; recombinant proteins; Recombinant Proteins - immunology; Reproducibility of Results; Streptococcal Infections - blood; Streptococcal Infections - diagnosis; Streptococcal Infections - genetics; Streptococcal Infections - immunology; Streptococcus pyogenes; Streptococcus pyogenes - enzymology; Streptococcus pyogenes - genetics; Streptococcus pyogenes - immunology; streptolysin; Time Factors; His-tagged DNase B; SCPA; dnaseB; Group A Streptococcus; Streptococcus pyogenes; DNA methyl green micromethod; ASO; PCR; DMGM; ADB; ELISA

Among the four known Streptococcal nucleases comprising of DNase A, B, C and D; DNase B is the most common, and determination of the levels of antibody to DNase B (ADB) is often used to confirm a clinical diagnosis of Streptococcus pyogenes/group A Streptococcal (GAS) infection. The commonly used as...