Intracellular transformation of prolactin following internalization into rat liver
We investigated prolactin (PRL) degradation in rat liver lysosomes both in vivo and in vitro. In previous studies we showed that, in addition to the Golgi apparatus, PRL is internalized towards lysosomes and light, lysosome-like vesicles which we identified as ' prelysosomes'. Injected [ 1...
Saved in:
Published in | Molecular and cellular endocrinology Vol. 35; no. 1; pp. 25 - 31 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
01.04.1984
Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | We investigated prolactin (PRL) degradation in rat liver lysosomes both in vivo and in vitro. In previous studies we showed that, in addition to the Golgi apparatus, PRL is internalized towards lysosomes and light, lysosome-like vesicles which we identified as ' prelysosomes'. Injected [
125I]oPRL that localized in lysosomes and prelysosomes at times varying from 0 to 45 min showed significant differences from fresh and plasma membrane- (PM) or Golgi-bound hormone. First, it was more easily dissociable by 3 M MgCl
2 than Golgi- but less than PM-bound [
125I]oPRL. Second, it was only in lysosomal fractions that, as time following injection increased, a significant part of dissociable radioactivity became non-TAC-precipitable. When MgCl
2-extracted [
125I]oPRL was subjected to gel filtration on a Sephadex G-75 fine column, some of the radioactivity, and especially that extracted from prelysosomal or lysosomal fractions, eluted as a high molecular weight (HMW) entity, most co-migrated with fresh [
125I]oPRL, and a little was found in small fragments. Only the central peak had any rebinding activity, which was comparable to that of fresh hormone.
In an in vitro study we incubated [
125I]hGH with lysosomal fractions for 16 h at 25°C. After centrifugation, an aliquot of supernatant hormone was assayed for its binding capacity to standard receptor preparations and the rest subjected to gel filtration. Peak fractions were also tested in binding assay. [
125I]hGH that had been in contact with prelysosomes lost almost all of its ability to bind to standard receptors and totally migrated in the HMW peak, at the void volume of the column. Hormone incubated with lysosomes bound about 65% as well as the fresh hormone and, correspondingly, 35–40% of the radioactivity was found in the HMW peak and a little as small fragments. It is possible that in lysosomes, and especially in prelysosomes, binding of the hormone to its receptor becomes irreversible and the hormone-receptor complex may become partially solubilized. |
---|---|
AbstractList | We investigated prolactin (PRL) degradation in rat liver lysosomes both in vivo and in vitro. In previous studies we showed that, in addition to the Golgi apparatus, PRL is internalized towards lysosomes and light, lysosome-like vesicles which we identified as 'prelysosomes'. Injected [125I]oPRL that localized in lysosomes and prelysosomes at times varying from 0 to 45 min showed significant differences from fresh and plasma membrane- (PM) or Golgi-bound hormone. First, it was more easily dissociable by 3 M MgCl2 than Golgi- but less than PM-bound [125I]oPRL. Second, it was only in lysosomal fractions that, as time following injection increased, a significant part of dissociable radioactivity became non-TAC-precipitable. When MgCl2-extracted [125I]oPRL was subjected to gel filtration on a Sephadex G-75 fine column, some of the radioactivity, and especially that extracted from prelysosomal or lysosomal fractions, eluted as a high molecular weight (HMW) entity, most co-migrated with fresh [125I]oPRL, and a little was found in small fragments. Only the central peak had any rebinding activity, which was comparable to that of fresh hormone. In an in vitro study we incubated [125I]hGH with lysosomal fractions for 16 h at 25 degrees C. After centrifugation, an aliquot of supernatant hormone was assayed for its binding capacity to standard receptor preparations and the rest subjected to gel filtration. Peak fractions were also tested in binding assay. [125I]hGH that had been in contact with prelysosomes lost almost all of its ability to bind to standard receptors and totally migrated in the HMW peak, at the void volume of the column. We investigated prolactin (PRL) degradation in rat liver lysosomes both in vivo and in vitro. In previous studies we showed that, in addition to the Golgi apparatus, PRL is internalized towards lysosomes and light, lysosome-like vesicles which we identified as ' prelysosomes'. Injected [ 125I]oPRL that localized in lysosomes and prelysosomes at times varying from 0 to 45 min showed significant differences from fresh and plasma membrane- (PM) or Golgi-bound hormone. First, it was more easily dissociable by 3 M MgCl 2 than Golgi- but less than PM-bound [ 125I]oPRL. Second, it was only in lysosomal fractions that, as time following injection increased, a significant part of dissociable radioactivity became non-TAC-precipitable. When MgCl 2-extracted [ 125I]oPRL was subjected to gel filtration on a Sephadex G-75 fine column, some of the radioactivity, and especially that extracted from prelysosomal or lysosomal fractions, eluted as a high molecular weight (HMW) entity, most co-migrated with fresh [ 125I]oPRL, and a little was found in small fragments. Only the central peak had any rebinding activity, which was comparable to that of fresh hormone. In an in vitro study we incubated [ 125I]hGH with lysosomal fractions for 16 h at 25°C. After centrifugation, an aliquot of supernatant hormone was assayed for its binding capacity to standard receptor preparations and the rest subjected to gel filtration. Peak fractions were also tested in binding assay. [ 125I]hGH that had been in contact with prelysosomes lost almost all of its ability to bind to standard receptors and totally migrated in the HMW peak, at the void volume of the column. Hormone incubated with lysosomes bound about 65% as well as the fresh hormone and, correspondingly, 35–40% of the radioactivity was found in the HMW peak and a little as small fragments. It is possible that in lysosomes, and especially in prelysosomes, binding of the hormone to its receptor becomes irreversible and the hormone-receptor complex may become partially solubilized. |
Author | Djiane, Jean Kelly, Paul A. Ferland, Louis H. Houdebine, Louis-M. |
Author_xml | – sequence: 1 givenname: Louis H. surname: Ferland fullname: Ferland, Louis H. organization: Department of Molecular Endocrinology, Le Centre Hospitalier de l'Université Lavai, Québec, GI V 4G2 Canada – sequence: 2 givenname: Jean surname: Djiane fullname: Djiane, Jean organization: Laboratoire de Physiologie de la Lactation, Institut National de Recherches Agronomiques, 78350, Jouy-en-JosasFrance – sequence: 3 givenname: Louis-M. surname: Houdebine fullname: Houdebine, Louis-M. organization: Laboratoire de Physiologie de la Lactation, Institut National de Recherches Agronomiques, 78350, Jouy-en-JosasFrance – sequence: 4 givenname: Paul A. surname: Kelly fullname: Kelly, Paul A. organization: Department of Molecular Endocrinology, Le Centre Hospitalier de l'Université Lavai, Québec, GI V 4G2 Canada |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9649258$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/6325276$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kEtLJDEQgIO46IzuP1DowyJ6aDeP7iRzEUR8gbAg6zlUpytLlkziJj3K7q83szPM0VNVUV8VVd-c7McUkZATRi8ZZfI7FVS0ilN1rruLBaVctmyPzJhWvNW0V_tktkMOybyU35RS1XN9QA6k4D1XckaeH-OUwWIIqwC5qXksLuUlTD7FJrnmNacAdvKxcSmE9O7jr8bHCXOE4P9tsFqnJsPUBP-G-Zh8cRAKft3GI_Jyd_vz5qF9-nH_eHP91Fqh5dRK6Rx0gvNuQKY7Aba3o3OM9sxhD4AgB6nUuBg6p-1gtVR0cFIqoVAAjOKInG321hP_rLBMZunL-hOImFbFaEa5FqqvYLcBbU6lZHTmNfsl5L-GUbNWadaezNqT0Z35r9KwOna63b8aljjuhrbuav_btg_FQnBVnfVlhy1kt-C9rtjVBsPq4s1jNsV6jBZHn9FOZkz-8zs-ACjZk6A |
CODEN | MCEND6 |
CitedBy_id | crossref_primary_10_1016_0955_2863_93_90077_A crossref_primary_10_1016_0024_3205_87_90096_8 crossref_primary_10_1242_jcs_84_1_19 |
Cites_doi | 10.1016/S0021-9258(18)34073-0 10.1210/endo-104-2-372 10.1210/edrv-2-2-137 10.1083/jcb.78.2.349 10.1016/S0021-9258(17)30293-4 10.1210/endo-97-6-1408 10.1016/S0021-9258(19)69476-7 10.1210/endo-95-2-521 10.1016/S0021-9258(18)33706-2 10.1083/jcb.93.1.82 10.1210/edrv-3-3-280 10.1016/S0021-9258(19)70425-6 10.1083/jcb.59.1.73 10.1210/endo-104-6-1631 10.1042/bj0600604 10.1016/0005-2736(70)90159-8 10.1016/S0021-9258(19)86342-1 10.1210/endo-106-1-179 10.1016/S0021-9258(17)34798-1 10.2337/diab.28.5.460 10.1016/S0021-9258(19)52451-6 |
ContentType | Journal Article |
Copyright | 1984 1984 INIST-CNRS |
Copyright_xml | – notice: 1984 – notice: 1984 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1016/0303-7207(84)90026-1 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Anatomy & Physiology |
EISSN | 1872-8057 |
EndPage | 31 |
ExternalDocumentID | 10_1016_0303_7207_84_90026_1 6325276 9649258 0303720784900261 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K --M -~X .55 .GJ .~1 0R~ 123 1B1 1RT 1~. 1~5 29M 3O- 4.4 457 4G. 53G 5RE 5VS 7-5 71M 8P~ 9JM AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO ABBQC ABFNM ABFRF ABGSF ABJNI ABLVK ABMAC ABMZM ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CS3 DOVZS DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HDZ HLW HMK HMO HVGLF HZ~ IHE J1W J5H KOM LCYCR LX3 LZ1 M29 M41 MO0 MVM N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SAE SBG SCC SCU SDF SDG SDP SES SEW SPCBC SSH SSU SSZ T5K WH7 WUQ X7M ZGI ZXP ~G- AALMO AAPBV ABFLS ABPIF ABPTK ABQIS ADALY ADGIM IPNFZ IQODW AAHBH AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c386t-66ffa43224be1843ac5cdff1051fe5aaea6b677d9b4f8cbc8670bf66737e3aad3 |
ISSN | 0303-7207 |
IngestDate | Fri Aug 16 06:11:35 EDT 2024 Thu Sep 26 16:17:11 EDT 2024 Sat Sep 28 08:38:51 EDT 2024 Fri Nov 25 06:01:47 EST 2022 Fri Feb 23 02:20:30 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | prolactin processing prelysosome hormone-receptor complex intracellular receptors Golgi complex lysosome Cell organelle Rat Rodentia Lysosome Gonadotropin Biological activity Golgi apparatus Processing Protein hormone Vertebrata Mammalia Hepatocyte Prolactin Hormone receptor complex Intracellular Hormonal receptor |
Language | English |
License | CC BY 4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c386t-66ffa43224be1843ac5cdff1051fe5aaea6b677d9b4f8cbc8670bf66737e3aad3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 6325276 |
PQID | 81028375 |
PQPubID | 23479 |
PageCount | 7 |
ParticipantIDs | proquest_miscellaneous_81028375 crossref_primary_10_1016_0303_7207_84_90026_1 pubmed_primary_6325276 pascalfrancis_primary_9649258 elsevier_sciencedirect_doi_10_1016_0303_7207_84_90026_1 |
PublicationCentury | 1900 |
PublicationDate | 1984-Apr |
PublicationDateYYYYMMDD | 1984-04-01 |
PublicationDate_xml | – month: 04 year: 1984 text: 1984-Apr |
PublicationDecade | 1980 |
PublicationPlace | Shannon |
PublicationPlace_xml | – name: Shannon – name: Ireland |
PublicationTitle | Molecular and cellular endocrinology |
PublicationTitleAlternate | Mol Cell Endocrinol |
PublicationYear | 1984 |
Publisher | Elsevier Ireland Ltd Elsevier |
Publisher_xml | – name: Elsevier Ireland Ltd – name: Elsevier |
References | Olefsky, Kobayashi, Chang (BIB20) 1979; 28 Kelly, Djiane, Leblanc (BIB16) 1983; 172 Costlow, Hample (BIB6) 1982; 257 Posner, Khan, Bergeron (BIB25) 1982; 3 Bergeron, Posner, Josefsberg, Sikstrom (BIB2) 1978; 253 Josefsberg, Posner, Patel, Bergeron (BIB13) 1979; 254 Khan, Posner, Verma, Khan, Bergeron (BIB17) 1981; 78 Bergeron, Ehrenreich, Siekewitz, Palade (BIB1) 1973; 59 Grosvenor, Mena, Whitworth (BIB11) 1979; 104 Wattiaux, Wattiaux-De Coninck, Ronveaux-Dupal, Dubois (BIB27) 1978; 78 Ferland, L.H., Djiane, J., Houdebine, L.-M. and Kelly, P.A. Endocrinology (submitted). Posner, Kelly, Shiu, Friesen (BIB22) 1975; 95 Donner, Martin, Sonenberg (BIB8) 1978; 75 De Duve, Pressman, Gianetto, Wattiaux, Appelmans (BIB7) 1955; 60 Posner, Josefsberg, Bergeron (BIB23) 1979 Chertow (BIB4) 1981; 2 Clark, Harrison (BIB5) 1982; 257 Ray (BIB26) 1970; 196 Hizuka, Gorden, Lesniak, Van, Carpentier, Orci (BIB12) 1981; 256 Caro, Amatruda (BIB3) 1980; 255 Posner, Kelly, Friesen (BIB21) 1974; 71 Posner, Josefsberg, Bergeron (BIB24) 1979; 254 Krupp, Livingston (BIB18) 1980; 106 Kelly, Posner, Friesen (BIB14) 1975; 97 Kelly, Leblanc, Djiane (BIB15) 1979; 104 Lowry, Rosebrough, Farr, Randall (BIB19) 1951; 193 Widnell, Unkeless (BIB28) 1968; 68 Felhmann, Carpentier, LeCam, Thamm, Saunders, Brandenberg, Orci, Freychet (BIB9) 1982; 93 10.1016/0303-7207(84)90026-1_BIB10 Bergeron (10.1016/0303-7207(84)90026-1_BIB2) 1978; 253 Bergeron (10.1016/0303-7207(84)90026-1_BIB1) 1973; 59 De Duve (10.1016/0303-7207(84)90026-1_BIB7) 1955; 60 Lowry (10.1016/0303-7207(84)90026-1_BIB19) 1951; 193 Posner (10.1016/0303-7207(84)90026-1_BIB25) 1982; 3 Kelly (10.1016/0303-7207(84)90026-1_BIB15) 1979; 104 Khan (10.1016/0303-7207(84)90026-1_BIB17) 1981; 78 Widnell (10.1016/0303-7207(84)90026-1_BIB28) 1968; 68 Caro (10.1016/0303-7207(84)90026-1_BIB3) 1980; 255 Grosvenor (10.1016/0303-7207(84)90026-1_BIB11) 1979; 104 Felhmann (10.1016/0303-7207(84)90026-1_BIB9) 1982; 93 Olefsky (10.1016/0303-7207(84)90026-1_BIB20) 1979; 28 Chertow (10.1016/0303-7207(84)90026-1_BIB4) 1981; 2 Posner (10.1016/0303-7207(84)90026-1_BIB22) 1975; 95 Posner (10.1016/0303-7207(84)90026-1_BIB21) 1974; 71 Ray (10.1016/0303-7207(84)90026-1_BIB26) 1970; 196 Posner (10.1016/0303-7207(84)90026-1_BIB23) 1979 Costlow (10.1016/0303-7207(84)90026-1_BIB6) 1982; 257 Hizuka (10.1016/0303-7207(84)90026-1_BIB12) 1981; 256 Donner (10.1016/0303-7207(84)90026-1_BIB8) 1978; 75 Josefsberg (10.1016/0303-7207(84)90026-1_BIB13) 1979; 254 Krupp (10.1016/0303-7207(84)90026-1_BIB18) 1980; 106 Wattiaux (10.1016/0303-7207(84)90026-1_BIB27) 1978; 78 Kelly (10.1016/0303-7207(84)90026-1_BIB14) 1975; 97 Kelly (10.1016/0303-7207(84)90026-1_BIB16) 1983; 172 Clark (10.1016/0303-7207(84)90026-1_BIB5) 1982; 257 Posner (10.1016/0303-7207(84)90026-1_BIB24) 1979; 254 |
References_xml | – volume: 257 start-page: 12239 year: 1982 end-page: 12244 ident: BIB5 publication-title: J. Biol. Chem. contributor: fullname: Harrison – volume: 75 start-page: 672 year: 1978 end-page: 676 ident: BIB8 publication-title: Proc. Natl. Acad. Sci. (U.S.A.) contributor: fullname: Sonenberg – volume: 196 start-page: 1 year: 1970 end-page: 9 ident: BIB26 publication-title: Biochim. Biophys. Acta contributor: fullname: Ray – volume: 60 start-page: 604 year: 1955 end-page: 617 ident: BIB7 publication-title: Biochem. J. contributor: fullname: Appelmans – volume: 78 start-page: 4980 year: 1981 end-page: 4984 ident: BIB17 publication-title: Proc. Natl. Acad. Sci. (U.S.A.) contributor: fullname: Bergeron – volume: 256 start-page: 4591 year: 1981 end-page: 4597 ident: BIB12 publication-title: J. Biol. Chem. contributor: fullname: Orci – start-page: 173 year: 1979 end-page: 181 ident: BIB23 publication-title: Clinical Neuroendocrinology: A Pathophysiological Approach contributor: fullname: Bergeron – volume: 95 start-page: 521 year: 1975 end-page: 531 ident: BIB22 publication-title: Endocrinology contributor: fullname: Friesen – volume: 172 start-page: 219 year: 1983 end-page: 224 ident: BIB16 publication-title: Proc. Soc. Exp. Biol. Med. contributor: fullname: Leblanc – volume: 28 start-page: 460 year: 1979 end-page: 471 ident: BIB20 publication-title: Diabetes contributor: fullname: Chang – volume: 254 start-page: 209 year: 1979 end-page: 214 ident: BIB13 publication-title: J. Biol. Chem. contributor: fullname: Bergeron – volume: 93 start-page: 82 year: 1982 end-page: 87 ident: BIB9 publication-title: J. Cell Biol. contributor: fullname: Freychet – volume: 104 start-page: 1631 year: 1979 end-page: 1638 ident: BIB15 publication-title: Endocrinology contributor: fullname: Djiane – volume: 253 start-page: 4058 year: 1978 end-page: 4066 ident: BIB2 publication-title: J. Biol. Chem. contributor: fullname: Sikstrom – volume: 59 start-page: 73 year: 1973 end-page: 88 ident: BIB1 publication-title: J. Cell Biol. contributor: fullname: Palade – volume: 106 start-page: 179 year: 1980 end-page: 184 ident: BIB18 publication-title: Endocrinology contributor: fullname: Livingston – volume: 97 start-page: 1408 year: 1975 end-page: 1415 ident: BIB14 publication-title: Endocrinology contributor: fullname: Friesen – volume: 104 start-page: 372 year: 1979 end-page: 376 ident: BIB11 publication-title: Endocrinology contributor: fullname: Whitworth – volume: 257 start-page: 9330 year: 1982 end-page: 9334 ident: BIB6 publication-title: J. Biol. Chem. contributor: fullname: Hample – volume: 68 start-page: 1050 year: 1968 end-page: 1057 ident: BIB28 publication-title: Biochemistry contributor: fullname: Unkeless – volume: 254 start-page: 12494 year: 1979 end-page: 12499 ident: BIB24 publication-title: J. Biol. Chem. contributor: fullname: Bergeron – volume: 255 start-page: 10052 year: 1980 end-page: 10055 ident: BIB3 publication-title: J. Biol. Chem. contributor: fullname: Amatruda – volume: 78 start-page: 349 year: 1978 end-page: 368 ident: BIB27 publication-title: J. Cell Biol. contributor: fullname: Dubois – volume: 3 start-page: 280 year: 1982 end-page: 298 ident: BIB25 publication-title: Endocr. Rev. contributor: fullname: Bergeron – volume: 2 start-page: 137 year: 1981 end-page: 173 ident: BIB4 publication-title: Endocr. Rev. contributor: fullname: Chertow – volume: 193 start-page: 265 year: 1951 end-page: 275 ident: BIB19 publication-title: J. Biol. Chem. contributor: fullname: Randall – volume: 71 start-page: 2407 year: 1974 end-page: 2411 ident: BIB21 publication-title: Proc. Natl. Acad. Sci. (U.S.A.) contributor: fullname: Friesen – volume: 257 start-page: 9330 year: 1982 ident: 10.1016/0303-7207(84)90026-1_BIB6 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)34073-0 contributor: fullname: Costlow – ident: 10.1016/0303-7207(84)90026-1_BIB10 – volume: 104 start-page: 372 year: 1979 ident: 10.1016/0303-7207(84)90026-1_BIB11 publication-title: Endocrinology doi: 10.1210/endo-104-2-372 contributor: fullname: Grosvenor – volume: 2 start-page: 137 year: 1981 ident: 10.1016/0303-7207(84)90026-1_BIB4 publication-title: Endocr. Rev. doi: 10.1210/edrv-2-2-137 contributor: fullname: Chertow – volume: 78 start-page: 349 year: 1978 ident: 10.1016/0303-7207(84)90026-1_BIB27 publication-title: J. Cell Biol. doi: 10.1083/jcb.78.2.349 contributor: fullname: Wattiaux – volume: 254 start-page: 209 year: 1979 ident: 10.1016/0303-7207(84)90026-1_BIB13 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)30293-4 contributor: fullname: Josefsberg – volume: 97 start-page: 1408 year: 1975 ident: 10.1016/0303-7207(84)90026-1_BIB14 publication-title: Endocrinology doi: 10.1210/endo-97-6-1408 contributor: fullname: Kelly – volume: 256 start-page: 4591 year: 1981 ident: 10.1016/0303-7207(84)90026-1_BIB12 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)69476-7 contributor: fullname: Hizuka – volume: 95 start-page: 521 year: 1975 ident: 10.1016/0303-7207(84)90026-1_BIB22 publication-title: Endocrinology doi: 10.1210/endo-95-2-521 contributor: fullname: Posner – volume: 257 start-page: 12239 year: 1982 ident: 10.1016/0303-7207(84)90026-1_BIB5 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)33706-2 contributor: fullname: Clark – volume: 93 start-page: 82 year: 1982 ident: 10.1016/0303-7207(84)90026-1_BIB9 publication-title: J. Cell Biol. doi: 10.1083/jcb.93.1.82 contributor: fullname: Felhmann – volume: 3 start-page: 280 year: 1982 ident: 10.1016/0303-7207(84)90026-1_BIB25 publication-title: Endocr. Rev. doi: 10.1210/edrv-3-3-280 contributor: fullname: Posner – volume: 255 start-page: 10052 year: 1980 ident: 10.1016/0303-7207(84)90026-1_BIB3 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)70425-6 contributor: fullname: Caro – volume: 172 start-page: 219 year: 1983 ident: 10.1016/0303-7207(84)90026-1_BIB16 contributor: fullname: Kelly – volume: 78 start-page: 4980 year: 1981 ident: 10.1016/0303-7207(84)90026-1_BIB17 contributor: fullname: Khan – volume: 59 start-page: 73 year: 1973 ident: 10.1016/0303-7207(84)90026-1_BIB1 publication-title: J. Cell Biol. doi: 10.1083/jcb.59.1.73 contributor: fullname: Bergeron – volume: 104 start-page: 1631 year: 1979 ident: 10.1016/0303-7207(84)90026-1_BIB15 publication-title: Endocrinology doi: 10.1210/endo-104-6-1631 contributor: fullname: Kelly – start-page: 173 year: 1979 ident: 10.1016/0303-7207(84)90026-1_BIB23 contributor: fullname: Posner – volume: 75 start-page: 672 year: 1978 ident: 10.1016/0303-7207(84)90026-1_BIB8 contributor: fullname: Donner – volume: 68 start-page: 1050 year: 1968 ident: 10.1016/0303-7207(84)90026-1_BIB28 publication-title: Biochemistry contributor: fullname: Widnell – volume: 60 start-page: 604 year: 1955 ident: 10.1016/0303-7207(84)90026-1_BIB7 publication-title: Biochem. J. doi: 10.1042/bj0600604 contributor: fullname: De Duve – volume: 196 start-page: 1 year: 1970 ident: 10.1016/0303-7207(84)90026-1_BIB26 publication-title: Biochim. Biophys. Acta doi: 10.1016/0005-2736(70)90159-8 contributor: fullname: Ray – volume: 254 start-page: 12494 year: 1979 ident: 10.1016/0303-7207(84)90026-1_BIB24 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)86342-1 contributor: fullname: Posner – volume: 71 start-page: 2407 year: 1974 ident: 10.1016/0303-7207(84)90026-1_BIB21 contributor: fullname: Posner – volume: 106 start-page: 179 year: 1980 ident: 10.1016/0303-7207(84)90026-1_BIB18 publication-title: Endocrinology doi: 10.1210/endo-106-1-179 contributor: fullname: Krupp – volume: 253 start-page: 4058 year: 1978 ident: 10.1016/0303-7207(84)90026-1_BIB2 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)34798-1 contributor: fullname: Bergeron – volume: 28 start-page: 460 year: 1979 ident: 10.1016/0303-7207(84)90026-1_BIB20 publication-title: Diabetes doi: 10.2337/diab.28.5.460 contributor: fullname: Olefsky – volume: 193 start-page: 265 year: 1951 ident: 10.1016/0303-7207(84)90026-1_BIB19 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)52451-6 contributor: fullname: Lowry |
SSID | ssj0007528 |
Score | 1.3192087 |
Snippet | We investigated prolactin (PRL) degradation in rat liver lysosomes both in vivo and in vitro. In previous studies we showed that, in addition to the Golgi... |
SourceID | proquest crossref pubmed pascalfrancis elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 25 |
SubjectTerms | Animals Biological and medical sciences Cell Membrane - metabolism Chromatography, Gel Female Fundamental and applied biological sciences. Psychology Golgi Apparatus - metabolism Golgi complex Growth Hormone - metabolism hormone-receptor complex Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis In Vitro Techniques intracellular receptors Liver - metabolism lysosome Lysosomes - metabolism Molecular Weight prelysosome Prolactin - analysis Prolactin - metabolism prolactin processing Rats Rats, Inbred Strains Receptors, Cell Surface - metabolism Receptors, Prolactin Vertebrates: endocrinology |
Title | Intracellular transformation of prolactin following internalization into rat liver |
URI | https://dx.doi.org/10.1016/0303-7207(84)90026-1 https://www.ncbi.nlm.nih.gov/pubmed/6325276 https://search.proquest.com/docview/81028375 |
Volume | 35 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLbKJtAkhGBjosDAD4BAU0Yujp0-VsBUKEVo2sSesJzEljZ1SbWlQvDrOb7ETaZVXF6ixlHsKt_n489H5_gg9IKJUFAmdUhjRAKi8jLIC-ByGRYCCJWq1NRYmn2hkxPy6TQ9HQy-d6KWlk1-UPy6Ma_kf1CFNsBVZ8n-A7K-U2iA34AvXAFhuP4Vxh-1Z1a73k0sadPRoFYFgnWc68QFHUw4n9c_bP6KPfbZ5V_q-3ofaLA_1xEaXa06ayvn2ty3dhhZlTWYmr4__tAmDZtd_irf4f35mQul9d7oSb0sZX5mWz-vfLFT6Wpef3Xe1dKm5mXdABaXgRUmAYttFdvWutrDSHoscqYyvdGCW2eC7wpkdkZe6jUTNotB1H0FsFhcGGRpEqcxu3aitlmj3ZNbaDNmoxQ255vj6dG3qV-rWWqq7_rh2uTKiL71ba8z8sYNv4Vuux7XyZi7C3EFk0vZqijrty1GvhzfR_fcvgOPLYkeoIGsttHOuBJNffETv8ImEthAuo3uzFzAxQ466lEM9ymGa4U9xbCnGL5GMX1fY6AYNhR7iE4OPxy_mwSuDEdQJBltAkqVEgQMP8mlrg4kirQolQJhHimZCiEFzSlj5SgnKitgplMW5kqXk2UyEaJMdtFGVVfyEcJJJCIwGozQkBBW0BGJCxCQsVAsj4ooG6Kg_ap8YU9b4W0YosaDazx4RrjBg0dDxNpPz51itEqQA53-8OZeDyk_3Ijq4zrhnzxvkeNgcPV3hglTL694ZiQ5S4do1wLqX3XUeLzuwRO0tZo2T9FGc7mUeyBpm_yZ4-VvBKCdoA |
link.rule.ids | 315,786,790,27957,27958 |
linkProvider | Library Specific Holdings |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Intracellular+transformation+of+prolactin+following+internalization+into+rat+liver&rft.jtitle=Molecular+and+cellular+endocrinology&rft.au=Ferland%2C+L+H&rft.au=Djiane%2C+J&rft.au=Houdebine%2C+L+M&rft.au=Kelly%2C+P+A&rft.date=1984-04-01&rft.issn=0303-7207&rft.volume=35&rft.issue=1&rft.spage=25&rft_id=info:doi/10.1016%2F0303-7207%2884%2990026-1&rft_id=info%3Apmid%2F6325276&rft.externalDocID=6325276 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0303-7207&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0303-7207&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0303-7207&client=summon |