Femtomole detection of amino acids and dipeptides by gas chromatography—negative-ion chemical ionization mass spectrometry following alkylation with pentafluorobenzyl bromide

• Published in: Journal of chromatography. B, Biomedical applications Vol. 658; no. 2; pp. 213 - 221
• Main Authors: Márquez, Cristina D., Weintraub, Susan T., Smith, Philip C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.08.1994; Elsevier
• Subjects: Acylation; Alkylation; Amino Acid Sequence; Amino Acids - analysis; Analysis; Biological and medical sciences; Dipeptides - analysis; Fluorobenzenes; Gas Chromatography-Mass Spectrometry; General pharmacology; Indicators and Reagents; Medical sciences; Molecular Sequence Data; Oligopeptides - analysis; Pharmacology. Drug treatments; Urine; Biological fluid; Derivatization; Alkylation; Chemical ionization; Gas chromatography; Separation method; Analysis method; Aminoacid; Dipeptides; Negative ion; Mass spectrometry; Quantitative analysis

Amino acids and di- and tripeptides were derivatized by extractive alkylation using pentafluorobenzyl bromide (PFBBr) followed by reaction with heptafluorobutyric anhydride (HFBA) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). Good chromatographic separation and the formation of intense diag...