Functional involvement of protein kinase C, Rho-kinase and TRPC3 decreases while PLC increases with advancement of pregnancy in mediating oxytocin-induced myometrial contractions in water buffaloes (Bubalus bubalis)

• Published in: Theriogenology Vol. 92; pp. 176 - 189
• Main Authors: Sharma, Abhishek, Nakade, Udayraj P., Choudhury, Soumen, Garg, Satish Kumar
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2017
• Subjects: Animals; Buffalo myometrium; Buffaloes - physiology; Calcium Channel Blockers - pharmacology; Enzyme Inhibitors - administration & dosage; Enzyme Inhibitors - pharmacology; Female; Gene Expression Regulation, Enzymologic; Imidazoles - pharmacology; Indicators and Reagents - pharmacology; Myometrium - drug effects; Nifedipine - pharmacology; Oxytocin; Oxytocin - pharmacology; Phospholipase-C; PKC; Pregnancy; Protein Kinase C - metabolism; Pyrazoles - pharmacology; rho-Associated Kinases - metabolism; Rho-kinase; Ruthenium Red - pharmacology; Store-operated Ca2+ channel/transient receptor potential channel; TRPC Cation Channels - genetics; TRPC Cation Channels - metabolism; Type C Phospholipases - genetics; Type C Phospholipases - metabolism; Uterine Contraction - drug effects; Uterine Contraction - physiology; Vasodilator Agents - administration & dosage; Vasodilator Agents - pharmacology; Phospholipase-C; Buffalo myometrium; Rho-kinase; PKC; Store-operated Ca2+ channel/transient receptor potential channel; Oxytocin; Store-operated Ca(2+) channel/transient receptor potential channel

Present study unravels the involvement of different calcium signaling pathways in oxytocin-induced contractions in myometrium of non-pregnant and pregnant buffaloes during early and mid-pregnancy stages. Uteri of pregnant animals were more sensitive than of non-pregnant buffaloes. Phasic contraction...