Detection of cyprinid herpesvirus 2 by loop-mediated isothermal amplification in combination with a lateral flow dipstick

• Published in: Molecular and cellular probes Vol. 50; p. 101507
• Main Authors: Li, Yanli, Lin, Feng, Sun, Lihui, Huang, Aixia, Chen, Jianming, Hao, Guijie, Yuan, Xuemei, Zhang, Haiqi, Su, Shengqi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2020
• Subjects: Animals; Carassius; Carassius auratus (L.); CyHV-2 detection; Cyprinid herpesvirus 2; Cyprinidae - virology; detection limit; DNA; fish diseases; Fish Diseases - virology; goldfish; Herpesviridae - genetics; Herpesviridae - isolation & purification; LAMP-LFD; loop-mediated isothermal amplification; Molecular Diagnostic Techniques - methods; mortality; necrosis; Nucleic Acid Amplification Techniques - methods; PCR; polymerase chain reaction; Rheology - methods; Sensitivity and Specificity; Carassius auratus (L.); CyHV-2 detection; LAMP-LFD; PCR

We developed a convenient technique to detect Herpesviral haematopoietic necrosis attributed to cyprinid herpes virus 2 (CyHV-2), a serious disease of Crucian carp and goldfish related to high mortality. In the present study, we employed a lateral flow dipstick (LAMP-LFD) to present a loop-mediated...