Direct comparison of QIAamp DSP Virus Kit and QIAamp Circulating Nucleic Acid Kit regarding cell-free fetal DNA isolation from maternal peripheral blood

• Published in: Molecular and cellular probes Vol. 43; pp. 13 - 19
• Main Authors: Jain, Mark, Balatsky, Alexander Vladimirovich, Revina, Daria Borisovna, Samokhodskaya, Larisa Mikhailovna
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2019
• Subjects: analytical kits; blood; blood sampling; Cell-free fetal DNA; Cell-Free Nucleic Acids - blood; Cell-Free Nucleic Acids - isolation & purification; Digital PCR; DNA; DNA isolation; Female; Fetal DNA fraction; Fetus - metabolism; Humans; laboratory equipment; markets; Methylation-sensitive restriction; Molecular Diagnostic Techniques - methods; Polymerase Chain Reaction; Pregnancy; pregnant women; Prenatal; qualitative analysis; quantitative analysis; Reagent Kits, Diagnostic; viruses; Prenatal; Cell-free fetal DNA; Digital PCR; Methylation-sensitive restriction; Fetal DNA fraction; DNA isolation

Blood of pregnant women contains cell-free fetal DNA (cffDNA), which is widely used in non-invasive prenatal diagnosis. The modern laboratory equipment market provides huge variety of commercial kits for isolation of circulating nucleic acids, but unfortunately none of them are standardized for isol...