Structure of the repeating unit of the O-specific polysaccharide of the lipopolysaccharide of Yersinia kristensenii strain 490 (O:12,25)

• Published in: Carbohydrate research Vol. 228; no. 2; p. 415
• Main Authors: L'vov, V L, Gur'yanova, S V, Rodionov, A V, Gorshkova, R P
• Format: Journal Article
• Language: English
• Published: Netherlands 27.04.1992
• Subjects: Carbohydrate Sequence; Lipopolysaccharides - chemistry; Lipopolysaccharides - isolation & purification; Molecular Sequence Data; Species Specificity; Yersinia - chemistry
• DOI: 10.1016/0008-6215(92)84134-E
• ISSN: 0008-6215

The O-specific polysaccharide isolated by mild acid degradation of the lipopolysaccharide of Y. kristensenii strain 490 (O:12,25) contained D-glucose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, 2-acetamido-2,6-dideoxy-L-galactose, glycerol, and phosphate in the ratios 2:2:1:1:1:1. On the basis of 31P- and 13C-n.m.r. data, methylation analysis, dephosphorylation, solvolysis with anhydrous hydrogen fluoride, and Smith degradation, it was concluded that the repeating unit of the polysaccharide was a branched hexaosylglycerol phosphate with the following structure. [formula: see text]