Transcription at Early Stages of Herpes Simplex Virus 1 Infection and during Reactivation

• Published in: Intervirology Vol. 46; no. 1; pp. 25 - 34
• Main Authors: Rezuchova, Ingeborg, Kudelova, Marcela, Durmanova, Vladimira, Vojvodova, Andrea
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2003
• Subjects: Animals; Cercopithecus aethiops; Genes, Immediate-Early; Herpes simplex virus 1; Herpesvirus 1, Human - genetics; Herpesvirus 1, Human - physiology; ICP0 gene; ICP27 protein; ICP4 protein; Immediate-Early Proteins - genetics; Kinetics; Original Paper; Rabbits; RNA, Messenger - analysis; Thymidine Kinase - genetics; Transcription, Genetic; Ubiquitin-Protein Ligases; Vero Cells; Virus Activation; Virus Replication; Immediate early and early mRNA; Herpes simplex virus 1; Reactivation of latency; Transcription; RT-PCR

Objective: The kinetics of immediate early (IE) and early (E) herpes simplex virus 1 (HSV-1) mRNA transcription was followed in explanted trigeminal ganglia from rabbits with established latency. Methods: The expression of IE and E mRNAs was first assessed in infected Vero cells by RT-PCR and then i...