Characterisation of the cellular substrates for transglutaminase in normal liver and hepatocellular carcinoma

• Published in: Biochimica et biophysica acta Vol. 1033; no. 1; pp. 57 - 64
• Main Authors: Hand, David, Elliott, Barry M., Griffin, Martin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 29.01.1990; Elsevier; North-Holland
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Calcium Chloride - pharmacology; Cytosol - enzymology; Dipeptides - metabolism; Egtazic Acid - pharmacology; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Liver - enzymology; Liver Neoplasms, Experimental - enzymology; Male; Methylamine; Methylamines - metabolism; Molecular Weight; Polyamine; Polyamines - metabolism; Rats; Transferases; Transglutaminase; Transglutaminases - metabolism; Tumor growth; ϵ-(γ-Glutamyl)lysine; Polyamine; Methylamine; Transglutaminase; ϵ-(γ-Glutamyl)lysine; Tumor growth; Carcinoma; Radiolabelling; Rat; Gel electrophoresis; Enzyme; Liver; Reaction product; Rodentia; HPLC chromatography; Normal; Characterization; Substrate; Absorption spectrometry; Vertebrata; Mammalia; Enzymatic activity; Tumor; Protein-glutamine γ-glutamyltransferase; Comparative study

The transglutaminase-mediated incorporation of [ 14C]methylamine into tissue slices obtained from normal rat liver and diethylnitrosamine-induced hepatocellular carcinomas was used as a means of characterising the endogenous substrates of the transglutaminase enzymes present in these tissues. The am...