Rapid and effective detection of Macrobrachium rosenbergii nodavirus using a combination of nucleic acid sequence-based amplification test and immunochromatographic strip

• Published in: Journal of invertebrate pathology Vol. 198; p. 107921
• Main Authors: Lin, Feng, Shen, Jinyu, Liu, Yuelin, Huang, Aixia, Zhang, Haiqi, Chen, Fan, Zhou, Dongren, Zhou, Yang, Hao, Guijie
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2023
• Subjects: animal health; Animals; China; coat proteins; DNA; gene amplification; genes; human resources; Hybridization; immunoaffinity chromatography; infectious diseases; invertebrates; Lateral flow dipstick; Macrobrachium rosenbergii nodavirus; NASBA; Nodaviridae - genetics; Nucleic Acid Amplification Techniques - methods; Palaemonidae; RNA; RNA Viruses - genetics; Self-Sustained Sequence Replication; temperature; therapeutics; China; Macrobrachium rosenbergii nodavirus; Hybridization; NASBA; Lateral flow dipstick

[Display omitted] •The NASBA-LFD for detecting MrNV-chin was developed by amlification at 41 °C in a single tube within 90 min followed by hybridization.•The NASBA-LFD was specific for MrNV.•Sensitivity of NASBA-LFD was about 104 times higher in comparison to PCR-based detection.•The NASBA-LFD would...