Evidence for superoxide generation from the autoxidation of the favism-inducing aglycone divicine

• Published in: Biochimica et biophysica acta Vol. 926; no. 3; pp. 369 - 372
• Main Authors: Musci, Giovanni, Mavelli, Irene, Rotilio, Giuseppe
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07.12.1987; Elsevier; North-Holland
• Subjects: Aglycone; Analytical, structural and metabolic biochemistry; Autoxidation; Biological and medical sciences; Cyclic N-Oxides; Divicine; Electron Spin Resonance Spectroscopy; Enzymes and enzyme inhibitors; Favism; Fundamental and applied biological sciences. Psychology; Oxidation-Reduction; Oxidoreductases; Pentetic Acid; Pyrimidinones - metabolism; Superoxide production; Superoxides - metabolism; Autoxidation; DETAPAC; Divicine; Superoxide production; Aglycone; DMPO; Favism; TEMPO; Enzyme; Vicia faba; Glucose dehydrogenase; Hyperoxides; Reduction; Leguminosae; Dicotyledones; Angiospermae; Spermatophyta; Kinetics; EPR spectrum

The formation of the superoxide anion radical (O 2 −) during the autoxidation of divicine, an unstable aglycone involved in the hemolytic anemia occuring in favism, has been demonstrated by EPR with two different procedures. In the first case (chemical method) an O 2 −-mediated reduction of a nitrox...