Heparin removal by ecteola-cellulose pre-treatment enables the use of plasma samples for accurate measurement of anti-Yellow fever virus neutralizing antibodies

• Published in: Journal of immunological methods Vol. 448; pp. 9 - 20
• Main Authors: Campi-Azevedo, Ana Carolina, Peruhype-Magalhães, Vanessa, Coelho-dos-Reis, Jordana Grazziela, Costa-Pereira, Christiane, Yamamura, Anna Yoshida, Lima, Sheila Maria Barbosa de, Simões, Marisol, Campos, Fernanda Magalhães Freire, de Castro Zacche Tonini, Aline, Lemos, Elenice Moreira, Brum, Ricardo Cristiano, de Noronha, Tatiana Guimarães, Freire, Marcos Silva, Maia, Maria de Lourdes Sousa, Camacho, Luiz Antônio Bastos, Rios, Maria, Chancey, Caren, Romano, Alessandro, Domingues, Carla Magda, Teixeira-Carvalho, Andréa, Martins-Filho, Olindo Assis
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2017
• Subjects: Adolescent; Adult; Aged; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; anticoagulants; Anticoagulants - blood; Biomarkers - blood; blood sampling; blood serum; Blood Specimen Collection - methods; Cellulose - analogs & derivatives; Cellulose - chemistry; Child; Child, Preschool; Drug Monitoring - methods; Ecteola-cellulose; Female; fever; Heparin; Heparin - blood; Humans; immune response; Infant; Male; Middle Aged; neutralization; Neutralization Tests; neutralizing antibodies; Predictive Value of Tests; PRNT; Reproducibility of Results; Vaccination; vaccine development; vaccines; Vaccinology; Viral Plaque Assay; viruses; Yellow Fever - blood; Yellow Fever - diagnosis; Yellow Fever - prevention & control; Yellow Fever - virology; Yellow Fever Vaccine - administration & dosage; Yellow fever virus; Yellow fever virus - immunology; Young Adult; Vaccinology; Heparin; PRNT; Ecteola-cellulose; Yellow fever virus

Technological innovations in vaccinology have recently contributed to bring about novel insights for the vaccine-induced immune response. While the current protocols that use peripheral blood samples may provide abundant data, a range of distinct components of whole blood samples are required and th...