Sequestration and Synthesis: The Source of Insulin in Cell Clusters Differentiated from Murine Embryonic Stem Cells

• Published in: Stem cells (Dayton, Ohio) Vol. 23; no. 7; pp. 862 - 867
• Main Authors: Paek, Hyun Joon, Morgan, Jeffrey R., Lysaght, Michael J.
• Format: Journal Article
• Language: English
• Published: Bristol John Wiley & Sons, Ltd 01.08.2005
• Subjects: Animals; Autoradiography; C-Peptide - chemistry; Cell Differentiation; Cell Proliferation; Cells, Cultured; Culture Techniques; Cysteine - metabolism; Diabetes; Diffusion; Electrophoresis, Polyacrylamide Gel; Embryo, Mammalian - cytology; Embryonic stem cell; Fibroblasts - metabolism; Glucose - metabolism; Glucose stimulation; Guinea Pigs; Immunohistochemistry; Immunoprecipitation; Immunostaining; Insulin; Insulin - metabolism; Insulin Secretion; Islets of Langerhans - cytology; Mice; Microscopy, Electron, Transmission; Peptides - chemistry; Radioisotopes - metabolism; Rats; RNA, Messenger - metabolism; Stem Cells - cytology

The source of insulin released from insulin‐releasing cell clusters (IRCCs) differentiated from embryonic stem cells remains unclear. Rajagopal et al. have suggested that IRCCs do not synthesize but secrete insulin that had been absorbed from media during the multistep protocol. We report here furth...