Prevention of cardiotoxicity of aflatoxin B1 via dietary supplementation of papaya fruit extracts in rats

• Published in: Cytotechnology (Dordrecht) Vol. 66; no. 2; pp. 327 - 334
• Main Authors: Mannaa, Fathia A., Abdel-Wahhab, Khaled G., Abdel-Wahhab, Mosaad A.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2014; Springer Nature B.V
• Subjects: Aflatoxin B1; Agricultural commodities; Animals; Antioxidants; Biochemistry; Biomedicine; Biotechnology; Cardiotoxicity; Chemistry; Chemistry and Materials Science; Creatine; Creatine kinase; Dehydrogenases; Dietary supplements; Enzymes; Ethanol; Flavonoids; Food contamination & poisoning; Fruits; Heart; Kinases; L-Lactate dehydrogenase; Laboratories; Metabolites; Na+/K+-exchanging ATPase; Nitric oxide; Original Research; Oxidative stress; Phenolic compounds; Potassium; Statistical analysis; Toxicity; Variance analysis; Cairo Egypt; United States > US; Egypt; Spain; Heart; Oxidative stress; Aflatoxin B; Papaya; Antioxidant
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-013-9579-x

The aim of the current study was to evaluate the cardioprotective ability of water (WE) and ethanolic (EE) papaya fruits extracts against cardiotoxicity induced by aflatoxin B 1 (AFB 1 ) in rats. Forty two female Sprague–Dawley rats were divided into six treatment groups and treated orally for 2 weeks as follow: control group, the group treated with WE (250 mg/kg b.w), the group treated with EE (250 mg/kg b.w), the group treated orally with AFB 1 (17 μg/kg b.w) and the groups treated orally with AFB 1 plus WE or EE. The results indicated that treatment with AFB 1 resulted in oxidative stress in the heart manifested by the marked increase in cardiac malondialdehyde and calcium levels accompanied with a significant decrease in cardiac total antioxidant capacity. Serum nitric oxide and sodium levels, lactate dehydrogenase and creatine kinase isoenzyme activities were significantly increased, whereas, cardiac Na + /K + -ATPase activity and serum potassium were insignificantly affected. Supplementation with WE or EE effectively ameliorated most of the changes induced by AFB 1 . It could be concluded that both extracts attenuated the oxidative stress induced in heart tissue by AFB 1 and WE was more pronounced due to the higher total phenolic contents than in the EE.