Near Infrared Autofluorescence Lifetime Imaging of Human Retinal Pigment Epithelium Using Adaptive Optics Scanning Light Ophthalmoscopy

• Published in: Investigative ophthalmology & visual science Vol. 65; no. 5; p. 27
• Main Authors: Kunala, Karteek, Tang, Janet A. H., Bowles Johnson, Kristen E., Huynh, Khang T., Parkins, Keith, Kim, Hye-Jin, Yang, Qiang, Sparrow, Janet R., Hunter, Jennifer J.
• Format: Journal Article
• Language: English
• Published: United States The Association for Research in Vision and Ophthalmology 01.05.2024
• Subjects: Adult; Animals; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; Female; Fluorescein Angiography - methods; Humans; Infrared Rays; Male; Mice; Middle Aged; Ophthalmoscopy - methods; Optical Imaging - methods; Reproducibility of Results; Retinal Pigment Epithelium - diagnostic imaging; Retinal Pigment Epithelium - metabolism; Visual Psychophysics and Physiological Optics; Young Adult

To demonstrate the first near-infrared adaptive optics fluorescence lifetime imaging ophthalmoscopy (NIR-AOFLIO) measurements in vivo of the human retinal pigment epithelial (RPE) cellular mosaic and to visualize lifetime changes at different retinal eccentricities. NIR reflectance and autofluoresce...