Dipeptide synthesis by l-amino acid ligase from Ralstonia solanacearum

• Published in: Biochemical and biophysical research communications Vol. 371; no. 3; pp. 536 - 540
• Main Authors: Kino, Kuniki, Nakazawa, Yuji, Yagasaki, Makoto
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.07.2008
• Subjects: Bacillus subtilis; Dipeptide; Dipeptides - biosynthesis; Dipeptides - chemistry; Escherichia coli - genetics; l-Amino acid α-ligase; Peptide Synthases - chemistry; Peptide Synthases - genetics; Peptide Synthases - isolation & purification; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - isolation & purification; Protein Conformation; Ralstonia solanacearum; Ralstonia solanacearum - enzymology; Ralstonia solanacearum - genetics; Substrate Specificity; Dipeptide; Ralstonia solanacearum; l-Amino acid α-ligase

Despite its utility, dipeptides have not been widely used due to the absence of an efficient manufacturing method. Recently, a novel method for effective production of dipeptides using l-amino acid α-ligase (Lal) is presented. Lal, which is only identified in Bacillus subtilis, catalyzes dipeptide s...