Entamoeba histolytica EhDEAD1 is a conserved DEAD-box RNA helicase with ATPase and ATP-dependent RNA unwinding activities

• Published in: Gene Vol. 414; no. 1; pp. 19 - 31
• Main Authors: López-Camarillo, César, de la Luz García-Hernández, María, Marchat, Laurence A., Luna-Arias, Juan P., Hernández de la Cruz, Olga, Mendoza, Leobardo, Orozco, Esther
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.05.2008
• Subjects: Adenosine Triphosphatases - metabolism; Adenosine Triphosphate - metabolism; Amino Acid Sequence; Amoebiasis; Animals; Conserved Sequence; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - metabolism; Electrophoretic Mobility Shift Assay; Entamoeba histolytica; Entamoeba histolytica - enzymology; Entamoeba histolytica - genetics; Entamoeba histolytica - growth & development; Genetic Complementation Test; Lasers; Molecular Sequence Data; Phylogeny; Posttranscriptional regulation; Protozoan parasite; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA metabolism; RNA, Messenger; RNA, Protozoan - genetics; RNA, Protozoan - metabolism; S Phase - physiology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Sequence Homology, Amino Acid; aa; CE; nt; MESG; Posttranscriptional regulation; bp; PNP; Amoebiasis; NE; IPTG; BLAST; Protozoan parasite; RNA metabolism

RNA helicases are widely conserved key enzymes that perform multiple functions in RNA metabolism. Here, we present the cloning, expression and functional characterization of the EhDEAD1 RNA helicase in the protozoan parasite Entamoeba histolytica. According to its primary structure, EhDEAD1 is evolu...