Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide

• Published in: Biosensors & bioelectronics Vol. 34; no. 1; pp. 227 - 231
• Main Authors: Fu, Xiuli, Chen, Lingxin, Li, Jinhua, Lin, Meng, You, Huiyan, Wang, Wenhai
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.04.2012; Elsevier
• Subjects: Biological and medical sciences; Biosensing Techniques - methods; Biotechnology; Cetrimonium Compounds - chemistry; Colorimetric detection; Colorimetry; Fundamental and applied biological sciences. Psychology; Gold - chemistry; Gold nanorods; Graphene oxide; Graphite - chemistry; Heparin; Heparin - chemistry; Heparin - isolation & purification; Nanotubes - chemistry; Sensitivity and Specificity; Surface Plasmon Resonance; Graphene oxide; Heparin; Gold nanorods; Colorimetric detection; Gold; Detector; Graphene; Color; Colorimetry; Detection
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2012.02.008

► Coupled using of graphene oxides (GO) and gold nanorods (AuNRs) is studied. ► Electrostatic interaction of GO–AuNRs creates strong self-assemble of AuNRs. ► The color of AuNRs is fading by using super color quenching capacity of GO. ► The high affinity between protamine and heparin is employed. ► Advantages of the systems: label-free, simplicity, rapidity and high sensitivity. A novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02–0.28μg/mL (R=0.9957), and a limit of detection was 5ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin.