Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits Signal End Joining

• Published in: The Journal of immunology (1950) Vol. 196; no. 7; pp. 3032 - 3042
• Main Authors: Neal, Jessica A, Xu, Yao, Abe, Masumi, Hendrickson, Eric, Meek, Katheryn
• Format: Journal Article
• Language: English
• Published: United States 01.04.2016
• Subjects: Animals; Ataxia Telangiectasia Mutated Proteins - deficiency; Ataxia Telangiectasia Mutated Proteins - genetics; Cell Line; DNA End-Joining Repair; DNA-Activated Protein Kinase - deficiency; Ectopic Gene Expression; Embryonic Stem Cells - metabolism; Fibroblasts - metabolism; Gene Expression; Gene Targeting; HEK293 Cells; Humans; Mice; Phenotype; V(D)J Recombination

Unlike most DNA-dependent protein kinase, catalytic subunit (DNA-PKcs)-deficient mouse cell strains, we show in the present study that targeted deletion of DNA-PKcs in two different human cell lines abrogates VDJ signal end joining in episomal assays. Although the mechanism is not well defined, DNA-...