A simple spectrophotometric method for estimating total glucosinolates in mustard de-oiled cake
Glucosinolates are anti-nutritional factors present abundantly in the seed meal fraction of oilseed Brassica species. They are found in varying levels among different genotypes. Those genotypes containing less than 30 µmol/g are considered low/zero glucosinolate type and are preferred for edible pur...
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Published in | International journal of food properties Vol. 20; no. 12; pp. 3274 - 3281 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Abingdon
Taylor & Francis
02.12.2017
Taylor & Francis Ltd |
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Abstract | Glucosinolates are anti-nutritional factors present abundantly in the seed meal fraction of oilseed Brassica species. They are found in varying levels among different genotypes. Those genotypes containing less than 30 µmol/g are considered low/zero glucosinolate type and are preferred for edible purposes due to low pungency. Twenty two different genotypes were taken for the analysis of glucosinolates by spectrophotometry. A regression model was obtained using Ordinary Least Square technique which predicted a formula. Total glucosinolates (µmol/g) = 1.40 + 118.86 × A
425
, where A
425
is the absorbance at 425 nm. The total glucosinolate content obtained by the prediction formula when compared with HPLC data showed a correlation coefficient of 0.942. This high correlation between the two data sets validated the developed methodology. This method also simplifies the estimation of total glucosinolates by excluding the use of HPLC or other sophisticated instruments. |
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AbstractList | Glucosinolates are anti-nutritional factors present abundantly in the seed meal fraction of oilseed Brassica species. They are found in varying levels among different genotypes. Those genotypes containing less than 30 µmol/g are considered low/zero glucosinolate type and are preferred for edible purposes due to low pungency. Twenty two different genotypes were taken for the analysis of glucosinolates by spectrophotometry. A regression model was obtained using Ordinary Least Square technique which predicted a formula. Total glucosinolates (µmol/g) = 1.40 + 118.86 × A425, where A425 is the absorbance at 425 nm. The total glucosinolate content obtained by the prediction formula when compared with HPLC data showed a correlation coefficient of 0.942. This high correlation between the two data sets validated the developed methodology. This method also simplifies the estimation of total glucosinolates by excluding the use of HPLC or other sophisticated instruments. Glucosinolates are anti-nutritional factors present abundantly in the seed meal fraction of oilseed Brassica species. They are found in varying levels among different genotypes. Those genotypes containing less than 30 µmol/g are considered low/zero glucosinolate type and are preferred for edible purposes due to low pungency. Twenty two different genotypes were taken for the analysis of glucosinolates by spectrophotometry. A regression model was obtained using Ordinary Least Square technique which predicted a formula. Total glucosinolates (µmol/g) = 1.40 + 118.86 × A 425 , where A 425 is the absorbance at 425 nm. The total glucosinolate content obtained by the prediction formula when compared with HPLC data showed a correlation coefficient of 0.942. This high correlation between the two data sets validated the developed methodology. This method also simplifies the estimation of total glucosinolates by excluding the use of HPLC or other sophisticated instruments. |
Author | Gurung, Bishal Mawlong, Ibandalin Sujith Kumar, M.S. Singh, Dhiraj Singh, K.H. |
Author_xml | – sequence: 1 givenname: Ibandalin surname: Mawlong fullname: Mawlong, Ibandalin email: iban02@gmail.com organization: Biochemistry Division, ICAR - Directorate of Rapeseed-Mustard Research – sequence: 2 givenname: M.S. surname: Sujith Kumar fullname: Sujith Kumar, M.S. organization: Biochemistry Division, ICAR - Directorate of Rapeseed-Mustard Research – sequence: 3 givenname: Bishal surname: Gurung fullname: Gurung, Bishal organization: ICAR - Indian Agricultural Statistics Research Institute – sequence: 4 givenname: K.H. surname: Singh fullname: Singh, K.H. organization: Biochemistry Division, ICAR - Directorate of Rapeseed-Mustard Research – sequence: 5 givenname: Dhiraj surname: Singh fullname: Singh, Dhiraj organization: Biochemistry Division, ICAR - Directorate of Rapeseed-Mustard Research |
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SubjectTerms | Correlation coefficients Genotypes Glucosinolates High-performance liquid chromatography Mustard Palladium method Prediction formula Pungent principles Seed meal Spectrophotometry Total glucosinolate |
Title | A simple spectrophotometric method for estimating total glucosinolates in mustard de-oiled cake |
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