An improved method for measuring catalase activity in biological samples

• Published in: Biology methods and protocols Vol. 9; no. 1; p. bpae015
• Main Authors: Hadwan, Mahmoud Hussein, Hussein, Marwah Jaber, Mohammed, Rawa M, Hadwan, Asad M, Saad Al-Kawaz, Hawraa, Al-Obaidy, Saba S M, Al Talebi, Zainab Abbas
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 2024
• Subjects: spectrophotometry; ferrous ammonium sulfate; Bland–Altman plot; microplate protocol; sulfosalicylic acid

Catalase (CAT) is an important enzyme that protects biomolecules against oxidative damage by breaking down hydrogen peroxide (H2O2) into water and oxygen. CAT is present in all aerobic microbes, animals, and plants. It is, however, absent from normal human urine but can be detected in pathological u...