Biochemical Differentiation of Gestational Compartments in the Midgestational Fetal Rabbit

• Published in: Fetal diagnosis and therapy Vol. 16; no. 5; pp. 289 - 293
• Main Authors: Devlieger, R., Gratacós, E., Wu, J., Ardon, H., Vereecken, A., Deprest, J.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.09.2001; S. Karger AG
• Subjects: Alkaline Phosphatase - analysis; Allantois - metabolism; Amniotic Fluid - chemistry; Analysis of complex biological substances; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Blood and biological fluids; Body Fluids - chemistry; Extraembryonic Membranes - metabolism; Female; Fetal Blood - chemistry; Fundamental and applied biological sciences. Psychology; gamma-Glutamyltransferase - analysis; Gestational Age; Potassium - analysis; Pregnancy; Progesterone - analysis; Proteins - analysis; Rabbits; Sodium - analysis; Fetal membranes; Rabbit; Amnion; Coelocentesis; Chorion; Coelomic cavity; Biochemical analysis; Allantoic fluid; Biochemistry; Lagomorpha; Experimental study; Coelomic fluid; Pregnancy; Vertebrata; Mammalia; Animal; Amniotic fluid; Fetus
• DOI: 10.1159/000053930
• ISSN: 1015-3837

Objectives: The fetal rabbit at midgestation is increasingly being used as a model in fetal diagnosis and therapy. In this study, we aimed to establish a reliable method for identification of the origin of sampled extra-embryonic fluids based on selected biochemical components. Methods: In 6 pregnant does at 22 days of gestation, 18 gestational sacs were sampled for amniotic, allantoic and exocoelomic fluid. These fluids, as well as matching maternal and fetal blood samples, were assayed for levels of sodium, potassium, chloride, bicarbonate, total protein, alkaline phosphatase, γ-glutamyl transferase and progesterone. Results: Levels of sodium and potassium were, respectively, lower and higher in the allantoic fluid when compared to other extra-embryonic spaces. Amniotic fluid had a significantly lower total protein content and higher level of alkaline phosphatase when compared to the exocoelomic fluid. Significant levels of progesterone could only be detected in maternal blood. Conclusions: In the midgestational rabbit, a combined assay of potassium, alkaline phosphatase and progesterone can determine the gestational cavity of origin of the sampled fluid. The obtained gradients for these markers suggest compartment-specific production and/or inter-cavity transfer mechanisms.