Alternative pre-mRNA splicing in Drosophila spliceosomal assembly factor RNP-4F during development

• Published in: Gene Vol. 371; no. 2; pp. 234 - 245
• Main Authors: Fetherson, Rebecca A., Strock, Stephen B., White, Kristen N., Vaughn, Jack C.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 26.04.2006
• Subjects: 5' Untranslated Regions; 5′-UTR; Alternative Splicing; Animals; Base Sequence; Blotting, Northern; cis-regulatory elements; DNA; Drosophila; Drosophila melanogaster - genetics; Intron evolution; Introns; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleoproteins - genetics; RNA Precursors - genetics; RNA, Messenger - genetics; rnp-4f gene; Sequence Homology, Nucleic Acid; Spliceosomes - genetics; Alternative splicing; U-snRNP; cis-regulatory elements; nt; EST; mRNA; RRM; TI no; bp; PRP proteins; NLS; 5′-UTR; RNP-4F; RT-PCR; TPR; poly(A +); cDNA; rnp-4f gene; BLAST; CTM; PCR; PTC; Intron evolution

The 5′- and 3′-UTR regions in pre-mRNAs play a variety of roles in controlling eukaryotic gene expression, including translational modulation. Here we report the results of a systematic study of alternative splicing in rnp-4f, which encodes a Drosophila spliceosomal assembly factor. We show that mos...