Development and Validation of a Highly Accurate Quantitative Real-Time PCR Assay for Diagnosis of Bacterial Vaginosis

• Published in: Journal of clinical microbiology Vol. 54; no. 4; pp. 1017 - 1024
• Main Authors: Hilbert, David W., Smith, William L., Chadwick, Sean G., Toner, Geoffrey, Mordechai, Eli, Adelson, Martin E., Aguin, Tina J., Sobel, Jack D., Gygax, Scott E.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.04.2016
• Subjects: Adolescent; Adult; Animals; Bacteriology; Female; Humans; Longitudinal Studies; Microbiological Techniques - methods; Middle Aged; Molecular Diagnostic Techniques - methods; Real-Time Polymerase Chain Reaction - methods; Sensitivity and Specificity; United States; Vaginosis, Bacterial - diagnosis; Young Adult; United States

Bacterial vaginosis (BV) is the most common gynecological infection in the United States. Diagnosis based on Amsel's criteria can be challenging and can be aided by laboratory-based testing. A standard method for diagnosis in research studies is enumeration of bacterial morphotypes of a Gram-st...