Hybridization in ssDNA films—a multi-technique spectroscopy study

• Published in: Physical chemistry chemical physics : PCCP Vol. 13; no. 34; pp. 15512 - 15522
• Main Authors: Howell, Caitlin, Zhao, Jianli, Koelsch, Patrick, Zharnikov, Michael
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 14.09.2011
• Subjects: Adenine - chemistry; Chemistry; DNA, Single-Stranded - chemistry; Exact sciences and technology; General and physical chemistry; Gold - chemistry; Nucleic Acid Hybridization; Oligonucleotides; Photoelectron Spectroscopy - methods; Sulfhydryl Compounds - chemistry; Surface physical chemistry; Thymine - chemistry; Monolayer; High resolution; Film; Thymine; Adenine; Immobilization; Sum frequency; X ray; Hybridization; Fine structure; Photoelectron spectrometry; Oligonucleotide; Interface; X ray absorption; X-ray photoelectron spectra

A combination of X-ray photoelectron spectroscopy (XPS), high-resolution XPS, near-edge X-ray absorption fine structure (NEXAFS) spectroscopy, and sum-frequency-generation (SFG) spectroscopy was used to monitor two types of ssDNA films on Au(111) before and after hybridization. As probe systems, fil...