Kinetics of immunoassays with particles as labels: effect of antibody coupling using dendrimers as linkers

• Published in: Analyst (London) Vol. 136; no. 12; pp. 2533 - 2541
• Main Authors: GUBALA, Vladimir, CREAN, Carol, NOONEY, Robert, HEARTY, Stephen, MCDONNELL, Barry, HEYDON, Katherine, O'KENNEDY, Richard, MACCRAITH, Brian D, WILLIAMS, David E
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 21.06.2011
• Subjects: Agglomeration; Analytical chemistry; Antibodies; Antigens; Chemistry; Dendrimers; Dendrimers - chemistry; Exact sciences and technology; Immunoassay - methods; Joining; Kinetics; Labels; Miscellaneous; Nanoparticles - chemistry; Nanostructure; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Single-Chain Antibodies - chemistry; Single-Chain Antibodies - genetics; Single-Chain Antibodies - immunology; Surface chemistry; Fold; Chemical analysis; Dyes; Nanoparticle; Use; Capture; Immunological method; Aggregation; Antigen; Chemistry; Parameter; Kinetics; Conjugation; Coupling; Limit; Diffusion coefficient; Comparative study; Single chain antibody; Signal to noise ratio

In this article, we report on poly(amidoamine) dendrimers (PAMAM) as coupling agents for recombinant single-chain (ScFv) antibodies to nanoparticle (NP) labels, for use in immunoassay. We present a simple theory for the kinetics of particle capture onto a surface by means of an antibody-antigen reac...