Structural and Functional Basis for an EBNA1 Hexameric Ring in Epstein-Barr Virus Episome Maintenance

• Published in: Journal of virology Vol. 91; no. 19
• Main Authors: Deakyne, Julianna S., Malecka, Kimberly A., Messick, Troy E., Lieberman, Paul M.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.10.2017
• Subjects: Binding Sites - genetics; Cell Line, Tumor; Crystallography, X-Ray; DNA Replication - genetics; DNA, Viral - genetics; DNA-Binding Proteins - genetics; Epstein-Barr Virus Nuclear Antigens - genetics; Epstein-Barr Virus Nuclear Antigens - metabolism; Genome and Regulation of Viral Gene Expression; HEK293 Cells; HeLa Cells; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - growth & development; Histones - metabolism; Humans; Multiprotein Complexes - metabolism; Origin Recognition Complex - genetics; Plasmids - genetics; Protein Structure, Tertiary; Replication Origin - genetics; Virus Replication - genetics; episome maintenance; DNA-binding domain; viral latency; oligomers; hexameric ring; EBNA1; Epstein-Barr virus; OriP; cooperative binding

Epstein-Barr virus (EBV) establishes a stable latent infection that can persist for the life of the host. EBNA1 is required for the replication, maintenance, and segregation of the latent episome, but the structural features of EBNA1 that confer each of these functions are not completely understood....