Nicotinamide N-methyltransferase expression and its association with phospho-Akt, p53 expression, and survival in high-grade endometrial cancer
Nicotinamide N-methyltransferase (NNMT) is an enzyme that is overexpressed in malignancies. NNMT expression has not been previously studied in endometrial cancer (EC). Increased phospho-Akt (pAkt) levels in response to NNMT overexpression have been reported in in vitro studies of different cancer ty...
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Published in | Turkish journal of medical sciences Vol. 49; no. 5; pp. 1547 - 1554 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Turkey
The Scientific and Technological Research Council of Turkey
24.10.2019
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Abstract | Nicotinamide N-methyltransferase (NNMT) is an enzyme that is overexpressed in malignancies. NNMT expression has not been previously studied in endometrial cancer (EC). Increased phospho-Akt (pAkt) levels in response to NNMT overexpression have been reported in in vitro studies of different cancer types. We assayed NNMT expression in primary and metastatic high-grade EC and investigated the relationship of NNMT with p53, pAkt, and survival.
NNMT, pAkt, and p53 expressions were assayed in 100 tissue samples of benign endometria, primary EC, and metastatic EC by immunohistochemistry.
The NNMT immunoreactivity score was significantly higher in primary high-grade EC than benign endometrial tissue (P = 0.001). NNMT expression in metastatic tissue was significantly higher than in primary cancer (P < 0.001). Metastatic stromal NNMT expression was significantly higher than that of the adjacent tumor and stroma adjacent to the primary tumor. p53 expression in the primary tumor showed a significant positive correlation with omental NNMT and pAkt expression. NNMT expression was also correlated with pAkt expression in metastatic tissue. NNMT overexpression in metastatic tissue was associated with decreased survival (P = 0.039).
This study suggests that NNMT may promote cancer progression and that NNMT overexpression is associated with aberrant p53 expression, pAkt, and poor survival. NNMT’s role in cancer progression could make it a target of EC therapy. |
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AbstractList | Nicotinamide N-methyltransferase (NNMT) is an enzyme that is overexpressed in malignancies. NNMT expression has not been previously studied in endometrial cancer (EC). Increased phospho-Akt (pAkt) levels in response to NNMT overexpression have been reported in in vitro studies of different cancer types. We assayed NNMT expression in primary and metastatic high-grade EC and investigated the relationship of NNMT with p53, pAkt, and survival.
NNMT, pAkt, and p53 expressions were assayed in 100 tissue samples of benign endometria, primary EC, and metastatic EC by immunohistochemistry.
The NNMT immunoreactivity score was significantly higher in primary high-grade EC than benign endometrial tissue (P = 0.001). NNMT expression in metastatic tissue was significantly higher than in primary cancer (P < 0.001). Metastatic stromal NNMT expression was significantly higher than that of the adjacent tumor and stroma adjacent to the primary tumor. p53 expression in the primary tumor showed a significant positive correlation with omental NNMT and pAkt expression. NNMT expression was also correlated with pAkt expression in metastatic tissue. NNMT overexpression in metastatic tissue was associated with decreased survival (P = 0.039).
This study suggests that NNMT may promote cancer progression and that NNMT overexpression is associated with aberrant p53 expression, pAkt, and poor survival. NNMT’s role in cancer progression could make it a target of EC therapy. |
Author | Harmankaya, İsmail Çelik, Çetin Uğraş, Serdar Akar, Serra |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31652035$$D View this record in MEDLINE/PubMed |
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Copyright | This work is licensed under a Creative Commons Attribution 4.0 International License. Copyright © 2019 The Author(s) 2019 |
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Keywords | type II endometrial cancer disease-specific survival Akt Nicotinamide N-methyltransferase p53 |
Language | English |
License | This work is licensed under a Creative Commons Attribution 4.0 International License. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited. |
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Snippet | Nicotinamide N-methyltransferase (NNMT) is an enzyme that is overexpressed in malignancies. NNMT expression has not been previously studied in endometrial... |
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SubjectTerms | Adult Aged Aged, 80 and over Endometrial Neoplasms - chemistry Endometrial Neoplasms - enzymology Endometrial Neoplasms - mortality Endometrial Neoplasms - pathology Female Humans Middle Aged Neoplasm Grading Nicotinamide N-Methyltransferase - analysis Nicotinamide N-Methyltransferase - biosynthesis Proto-Oncogene Proteins c-akt - analysis Proto-Oncogene Proteins c-akt - biosynthesis Retrospective Studies Survival Rate Tumor Suppressor Protein p53 - analysis Tumor Suppressor Protein p53 - biosynthesis |
Title | Nicotinamide N-methyltransferase expression and its association with phospho-Akt, p53 expression, and survival in high-grade endometrial cancer |
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