Development of an enzyme-linked immunosorbent assay for fentanyl and applications of fentanyl antibody-coated nanoparticles for sample preparation

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 41; no. 4; pp. 1332 - 1341
• Main Authors: Mao, Chi-Liang, Zientek, Keith D., Colahan, Patrick T., Kuo, Mei-Yueh, Liu, Chi-Ho, Lee, Kuo-Ming, Chou, Chi-Chung
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 16.06.2006; Elsevier Science
• Subjects: Adjuvants, Anesthesia - blood; Adjuvants, Anesthesia - metabolism; Adjuvants, Anesthesia - urine; Analysis; Analytical, structural and metabolic biochemistry; Animals; Anti-Bacterial Agents - urine; Antibodies - metabolism; Antibody-coated magnetic nanoparticles; Biological and medical sciences; Capillary electrophoresis; Cross Reactions; Electrophoresis, Capillary - methods; Enzyme immunoassay; Enzyme-Linked Immunosorbent Assay - methods; Fentanyl; Fentanyl - analogs & derivatives; Fentanyl - metabolism; Fentanyl - urine; Fundamental and applied biological sciences. Psychology; General pharmacology; Horses; Medical sciences; Nanostructures; Pharmacology. Drug treatments; Rabbits; Fentanyl; Antibody-coated magnetic nanoparticles; Enzyme immunoassay; Capillary electrophoresis; Agonist; Antibody; Nanoparticle; μ Opioid receptor; Development; Microparticle; Opiates; Narcotic analgesic; ELISA assay

A sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of fentanyl in serum and urine. The ELISA used an indirect competitive method produced by coating the plate with thyroglobulin conjugated with fentanyl hapten. Antibodies against fentanyl–hemocyanin were detected b...