Smurf2-Mediated Stabilization of DNA Topoisomerase IIα Controls Genomic Integrity

• Published in: Cancer research (Chicago, Ill.) Vol. 77; no. 16; pp. 4217 - 4227
• Main Authors: Emanuelli, Andrea, Borroni, Aurora P, Apel-Sarid, Liat, Shah, Pooja A, Ayyathan, Dhanoop Manikoth, Koganti, Praveen, Levy-Cohen, Gal, Blank, Michael
• Format: Journal Article
• Language: English
• Published: United States American Association for Cancer Research, Inc 15.08.2017
• Subjects: Animals; Antigens, Neoplasm - genetics; Antigens, Neoplasm - metabolism; Antineoplastic drugs; Antitumor agents; Cancer; Cell Line, Tumor; Chromatin; Chromosomes; Deoxyribonucleic acid; DNA; DNA topoisomerase; DNA topoisomerase (ATP-hydrolysing); DNA Topoisomerases, Type II - genetics; DNA Topoisomerases, Type II - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Etoposide; Etoposide - pharmacology; Genomic Instability; Heredity; Humans; Interphase - physiology; Mice; Mice, Knockout; Mitosis; Neoplasms - genetics; Neoplasms - metabolism; Proteasomes; Tissues; Ubiquitin; Ubiquitin-protein ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Ubiquitination

DNA topoisomerase IIα (Topo IIα) ensures genomic integrity and unaltered chromosome inheritance and serves as a major target of several anticancer drugs. Topo IIα function is well understood, but how its expression is regulated remains unclear. Here, we identify the E3 ubiquitin ligase Smurf2 as a p...