Biophysical properties of a synthetic transit peptide from wheat chloroplast ribulose 1,5-bisphosphate carboxylase

• Published in: Journal of peptide science Vol. 13; no. 4; pp. 245 - 252
• Main Authors: Ambroggio, Ernesto E., Austen, Brian, Fidelio, Gerardo D.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.04.2007
• Subjects: Biophysical Phenomena; Biophysics; chloroplast lipids; chloroplast transit peptide; Chloroplasts; FT-IR; langmuir monolayer; Lipids - chemistry; peptide lateral surface stability; peptide-lipid interaction; Peptides - chemical synthesis; Peptides - chemistry; Protein Conformation; Ribulose-Bisphosphate Carboxylase - chemistry; Sodium Dodecyl Sulfate - chemistry; Spectroscopy, Fourier Transform Infrared; Triticum - enzymology; Triticum aestivum

The surface properties of pure RuBisCo transit peptide (RTP) and its interaction with zwitterionic, anionic phospholipids and chloroplast lipids were studied by using the Langmuir monolayer technique. Pure RTP is able to form insoluble films and the observed surface parameters are compatible with an...