PCR primers for the estimation of contamination by seeds with normal cytoplasm in rapeseed lots bearing male-sterility-inducing Ogu-INRA cytoplasm
A polymerase chain reaction (PCR)‐based test was developed to detect seeds bearing the ‘so‐called’ normal rapeseed cytoplasm in seed lots with an OGU‐INRA type cytoplasm. The test is based on the amplification of the orfB region of male fertile rapeseed mitochondrial DNA (mtDNA). The amplification r...
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| Published in | Plant breeding Vol. 116; no. 4; pp. 390 - 392 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.09.1997
Blackwell Wiley |
| Subjects | |
| Online Access | Get full text |
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| Abstract | A polymerase chain reaction (PCR)‐based test was developed to detect seeds bearing the ‘so‐called’ normal rapeseed cytoplasm in seed lots with an OGU‐INRA type cytoplasm. The test is based on the amplification of the orfB region of male fertile rapeseed mitochondrial DNA (mtDNA). The amplification reaction uses total nucleic acids of young seedlings, extracted in bulk. After the sequencing of the orfB gene region in the normal Brassica mtDNA, primers were designed for its amplification by PCR. Although the specificity of amplification for the male fertile (mf) rapeseed cytoplasm is partly impaired by the presence of tiny amounts of this fragment in the mtDNA of male sterile (ms) plants, this test proved to be applicable for the estimation of the level of contamination in seed lots in reconstituted mixes as well as in real lots. |
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| AbstractList | A polymerase chain reaction (PCR)‐based test was developed to detect seeds bearing the ‘so‐called’ normal rapeseed cytoplasm in seed lots with an OGU‐INRA type cytoplasm. The test is based on the amplification of the orfB region of male fertile rapeseed mitochondrial DNA (mtDNA). The amplification reaction uses total nucleic acids of young seedlings, extracted in bulk. After the sequencing of the orfB gene region in the normal Brassica mtDNA, primers were designed for its amplification by PCR. Although the specificity of amplification for the male fertile (mf) rapeseed cytoplasm is partly impaired by the presence of tiny amounts of this fragment in the mtDNA of male sterile (ms) plants, this test proved to be applicable for the estimation of the level of contamination in seed lots in reconstituted mixes as well as in real lots. Abstract A polymerase chain reaction (PCR)‐based test was developed to detect seeds bearing the ‘so‐called’ normal rapeseed cytoplasm in seed lots with an OGU‐INRA type cytoplasm. The test is based on the amplification of the orfB region of male fertile rapeseed mitochondrial DNA (mtDNA). The amplification reaction uses total nucleic acids of young seedlings, extracted in bulk. After the sequencing of the orfB gene region in the normal Brassica mtDNA, primers were designed for its amplification by PCR. Although the specificity of amplification for the male fertile (mf) rapeseed cytoplasm is partly impaired by the presence of tiny amounts of this fragment in the mtDNA of male sterile (ms) plants, this test proved to be applicable for the estimation of the level of contamination in seed lots in reconstituted mixes as well as in real lots. |
| Author | Tinchant, C Budar, F Defrance, M.C |
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| Cites_doi | 10.1007/BF00334821 10.1007/BF00389421 10.1093/nar/14.24.9755 10.1105/tpc.3.10.1109 10.1093/nar/14.8.3605 10.1016/0168-9525(90)90125-P 10.1007/BF00279379 10.1007/BF00521276 10.1002/j.1460-2075.1987.tb04832.x 10.1051/agro:19900904 10.1007/BF00326293 |
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| Keywords | Impurity Primer Mitochondrial DNA Oil plant(vegetal) Contamination Polymerase chain reaction Cruciferae Dicotyledones Seed Angiospermae Brassica napus Spermatophyta Cytoplasmic male sterility COLZA CONTROLE CYTOPLASME OGU-INRA BIOLOGIE MOLECULAIRE |
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| References | Palmer, J. D., and L. A. Herbon, 1986: Tricircular mitochondrial genomes of Brassica and Raphanus: reversal of repeat configurations by inversion Nucl. Acids Res. 14, 9755-9765. Small, I. D., P. G. Isaac, and C. J. Leaver, 1987: Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize. EMBO J. 6, 865-869. Gualberto, J. M., G. Bonnard, L. Lamattina, and J.-M. Grienenberger, 1991: Expression of the wheat mitochondrial nad3-rps12 transcription unit: correlation between editing and mRNA maturation. Plant Cell 3, 1109-1120. Palmer, J. D., 1990: Contrasting modes and tempos of genome evolution in land plant organelles. Trends Genet. 6, 115-120. Pelletier, G. C. Primard, F. Vedel, P. Chétrit, R. Rémy, P. Rousselle, and M. Renard, 1983: Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol. Gen. Genet. 191, 244-250. Vedel, F., P. Chétrit, C. Mathieu, G. Pelletier, and C. Primard, 1986: Several different mitochondrial DNA regions are involved in inter-genomic recombination in Brassica napus cybrid plants. Curr. Genet. 11, 17-24. Hoheisel, J., and F. M. Pohl, 1986: Simplified preparation of unidirectional deletion clones. Nucl. Acids Res. 14, 3605. Bonhomme, S., F. Budar, M. Férault, and G. Pelletier, 1991: A 2.5 kb Nco1 fragment of Ogura radish mitochondrial DNA is correlated with cytoplasmic male sterility in Brassica cybrids. Curr. Genet. 19, 121-127. Vedel, F., C. Mathieu, P. Chétrit, G. Pelletier, and C. Primard, 1987: Mitochondrial DNA variation in cytoplasmic male sterile somatic hybrids of Brassica napus. Plant Physiol. Biochem. 25, 249-257. Bervillé, A., P. Faivre-Rampant, S. Santoni and E. Moreau, 1990: Controle de la pureté des semences de base et de prébase male-steriles cytoplasmiques par hybridation moléculaire avec un plasmide mitochondrial. Agronomic 10, 727-734. Bonhomme, S., F. Budar, D. Lancelin, I. Small, M.-C. Defrance, and G. Pelletier, 1992: Sequence and transcript analysis of Neo25 Ogura specific fragment correlated with cytoplasmic male sterility in Brassica cybrids. Mol. Gen. Genet. 235, 240-248. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl, 1990: Current Protocols in Molecular Biology. John Wiley and Sons, New York . Nugent, J. M., and J. D. Palmer, 1988: Location, identity, amount and serial entry of chloroplast DNA sequences in crucifer mitochondrial DNAs. Curr. Genet. 14, 501-509. Ogura, H., 1968: Studies on the new male sterility in Japanese radish, with special references to utilization of this sterility towards the practical raising of hybrid seeds. Mem. Fac. Agric. Kagoshima Univ. 6, 39-78. 1991; 3 1987; 25 1990; 10 1991; 19 1990 1986; 11 1988; 14 1992; 235 1986; 14 1983; 191 1987; 6 1968; 6 1990; 6 e_1_2_1_7_1 e_1_2_1_5_1 Vedel F. (e_1_2_1_15_1) 1987; 25 e_1_2_1_6_1 e_1_2_1_3_1 e_1_2_1_12_1 e_1_2_1_4_1 e_1_2_1_13_1 e_1_2_1_10_1 Ogura H. (e_1_2_1_8_1) 1968; 6 e_1_2_1_11_1 Ausubel F. M. (e_1_2_1_2_1) 1990 e_1_2_1_14_1 e_1_2_1_9_1 |
| References_xml | – volume: 6 start-page: 865 year: 1987 end-page: 869 article-title: Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize publication-title: EMBO J. – volume: 6 start-page: 39 year: 1968 end-page: 78 article-title: Studies on the new male sterility in Japanese radish, with special references to utilization of this sterility towards the practical raising of hybrid seeds publication-title: Mem. Fac. Agric. Kagoshima Univ. – volume: 191 start-page: 244 year: 1983 end-page: 250 article-title: Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion publication-title: Mol. Gen. Genet. – volume: 14 start-page: 3605 year: 1986 article-title: Simplified preparation of unidirectional deletion clones publication-title: Nucl. Acids Res. – volume: 235 start-page: 240 year: 1992 end-page: 248 article-title: Sequence and transcript analysis of Ogura specific fragment correlated with cytoplasmic male sterility in cybrids publication-title: Mol. Gen. Genet. – volume: 6 start-page: 115 year: 1990 end-page: 120 article-title: Contrasting modes and tempos of genome evolution in land plant organelles publication-title: Trends Genet – volume: 11 start-page: 17 year: 1986 end-page: 24 article-title: Several different mitochondrial DNA regions are involved in inter‐genomic recombination in cybrid plants publication-title: Curr. Genet. – volume: 3 start-page: 1109 year: 1991 end-page: 1120 article-title: Expression of the wheat mitochondrial nad3‐rps12 transcription unit: correlation between editing and mRNA maturation publication-title: Plant Cell – volume: 19 start-page: 121 year: 1991 end-page: 127 article-title: A 2.5 kb 1 fragment of Ogura radish mitochondrial DNA is correlated with cytoplasmic male sterility in cybrids publication-title: Curr. Genet. – volume: 14 start-page: 9755 year: 1986 end-page: 9765 article-title: Tricircular mitochondrial genomes of Brassica and Raphanus: reversal of repeat configurations by inversion Nucl publication-title: Acids Res. – volume: 10 start-page: 727 year: 1990 end-page: 734 article-title: Controle de la pureté des semences de base et de prébase male‐steriles cytoplasmiques par hybridation moléculaire avec un plasmide mitochondrial publication-title: Agronomic – volume: 14 start-page: 501 year: 1988 end-page: 509 article-title: Location, identity, amount and serial entry of chloroplast DNA sequences in crucifer mitochondrial DNAs publication-title: Curr. Genet. – year: 1990 – volume: 25 start-page: 249 year: 1987 end-page: 257 article-title: Mitochondrial DNA variation in cytoplasmic male sterile somatic hybrids of Brassica napus publication-title: Plant Physiol. Biochem. – ident: e_1_2_1_12_1 doi: 10.1007/BF00334821 – volume: 6 start-page: 39 year: 1968 ident: e_1_2_1_8_1 article-title: Studies on the new male sterility in Japanese radish, with special references to utilization of this sterility towards the practical raising of hybrid seeds publication-title: Mem. Fac. Agric. Kagoshima Univ. contributor: fullname: Ogura H. – ident: e_1_2_1_14_1 doi: 10.1007/BF00389421 – volume: 25 start-page: 249 year: 1987 ident: e_1_2_1_15_1 article-title: Mitochondrial DNA variation in cytoplasmic male sterile somatic hybrids of Brassica napus publication-title: Plant Physiol. Biochem. contributor: fullname: Vedel F. – ident: e_1_2_1_11_1 doi: 10.1093/nar/14.24.9755 – ident: e_1_2_1_6_1 doi: 10.1105/tpc.3.10.1109 – volume-title: Current Protocols in Molecular Biology year: 1990 ident: e_1_2_1_2_1 contributor: fullname: Ausubel F. M. – ident: e_1_2_1_7_1 doi: 10.1093/nar/14.8.3605 – ident: e_1_2_1_10_1 doi: 10.1016/0168-9525(90)90125-P – ident: e_1_2_1_5_1 doi: 10.1007/BF00279379 – ident: e_1_2_1_9_1 doi: 10.1007/BF00521276 – ident: e_1_2_1_13_1 doi: 10.1002/j.1460-2075.1987.tb04832.x – ident: e_1_2_1_3_1 doi: 10.1051/agro:19900904 – ident: e_1_2_1_4_1 doi: 10.1007/BF00326293 |
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| Snippet | A polymerase chain reaction (PCR)‐based test was developed to detect seeds bearing the ‘so‐called’ normal rapeseed cytoplasm in seed lots with an OGU‐INRA type... Abstract A polymerase chain reaction (PCR)‐based test was developed to detect seeds bearing the ‘so‐called’ normal rapeseed cytoplasm in seed lots with an... |
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| SubjectTerms | Agronomy. Soil science and plant productions Biological and medical sciences Brassica napus cytoplasmic male sterility detection DNA DNA primers estimation Fundamental and applied biological sciences. Psychology genes Genetics and breeding of economic plants genomics Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia Life Sciences male fertility male-sterility mitochondrial DNA Ogu-INRA cytoplasm orfB orfb gene PCR Plant breeding: fundamental aspects and methodology polymerase chain reaction seed contamination seeds Vegetal Biology |
| Title | PCR primers for the estimation of contamination by seeds with normal cytoplasm in rapeseed lots bearing male-sterility-inducing Ogu-INRA cytoplasm |
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