A Novel Tool for the Generation of Conditional Knockouts To Study Gene Function across the Plasmodium falciparum Life Cycle

• Published in: mBio Vol. 10; no. 5
• Main Authors: Tibúrcio, Marta, Yang, Annie S. P., Yahata, Kazuhide, Suárez-Cortés, Pablo, Belda, Hugo, Baumgarten, Sebastian, van de Vegte-Bolmer, Marga, van Gemert, Geert-Jan, van Waardenburg, Youri, Levashina, Elena A., Sauerwein, Robert W., Treeck, Moritz
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 17.09.2019
• Subjects: Gene Deletion; Gene Knockout Techniques; Genes, Protozoan; Host-Microbe Biology; Integrases - genetics; Life Cycle Stages - genetics; malaria; Molecular Biology - methods; molecular methods; Mosquito Vectors; Phenotype; Plasmodium falciparum; Plasmodium falciparum - enzymology; Plasmodium falciparum - genetics; reverse genetic analysis; Sirolimus; molecular methods; Plasmodium falciparum; reverse genetic analysis; malaria

One of the major limitations in studying P. falciparum is that so far only asexual stages are amenable to rapid conditional genetic modification. The most promising drug targets and vaccine candidates, however, have been refractory to genetic modification because they are essential during the blood...