LC-MS/MS assay for the simultaneous determination of tocopherols, polyunsaturated fatty acids and their metabolites in human plasma and serum

The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present,...

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Published in	Free radical biology & medicine Vol. 144; pp. 134 - 143
Main Authors	Giusepponi, Danilo, Galarini, Roberta, Barola, Carolina, Torquato, Pierangelo, Bartolini, Desirée, Moretti, Simone, Saluti, Giorgio, Gioiello, Antimo, Libetta, Carmelo, Galli, Francesco
Format	Journal Article
Language	English
Published	United States Elsevier Inc 20.11.2019
Subjects	Eicosanoids Human plasma/serum Liquid-chromatography tandem-mass spectrometry (LC-MS/MS) Polyunsaturated fatty acids Tocopheryl quinone Validation Vitamin E Validation Tocopheryl quinone Vitamin E Polyunsaturated fatty acids Eicosanoids Human plasma/serum Liquid-chromatography tandem-mass spectrometry (LC-MS/MS)
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ISSN	0891-5849 1873-4596 1873-4596
DOI	10.1016/j.freeradbiomed.2019.04.017

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Abstract	The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present, there are not procedures able to simultaneously determine these two classes of compounds. After the optimization of sample treatment and reverse-phase separation conditions, tandem mass spectrometry detection was evaluated experimenting both positive and negative electrospray ionisation modes. The procedure was also preliminarily adapted to assess five arachidonic acid-derived eicosanoids that could be under the influence of vitamin E function, such as LTB4 (leukotriene B4), 20-HETE (20-hydroxyeicosatetraenoic acid) and their ω-oxidation metabolites. After the validation study, the performance characteristics were confirmed analysing a certified reference material (SRM® 1950 - frozen human plasma by NIST). Finally, an application of the method in the analysis of lipid abnormalities of chronic kidney disease patients was shown. [Display omitted] •Simultaneous determination of vitamins E, PUFAs and metabolites in blood.•Satisfactory validation data were obtained.•Quality assurance issues have been stressed analyzing a CRM (plasma).•A stability study was carried out at −20 °C and −80 °C for four months.•Preliminary data evidenced differences between healthy subjects and renal patients.
AbstractList	The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present, there are not procedures able to simultaneously determine these two classes of compounds. After the optimization of sample treatment and reverse-phase separation conditions, tandem mass spectrometry detection was evaluated experimenting both positive and negative electrospray ionisation modes. The procedure was also preliminarily adapted to assess five arachidonic acid-derived eicosanoids that could be under the influence of vitamin E function, such as LTB4 (leukotriene B4), 20-HETE (20-hydroxyeicosatetraenoic acid) and their ω-oxidation metabolites. After the validation study, the performance characteristics were confirmed analysing a certified reference material (SRM® 1950 - frozen human plasma by NIST). Finally, an application of the method in the analysis of lipid abnormalities of chronic kidney disease patients was shown. [Display omitted] •Simultaneous determination of vitamins E, PUFAs and metabolites in blood.•Satisfactory validation data were obtained.•Quality assurance issues have been stressed analyzing a CRM (plasma).•A stability study was carried out at −20 °C and −80 °C for four months.•Preliminary data evidenced differences between healthy subjects and renal patients. The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present, there are not procedures able to simultaneously determine these two classes of compounds. After the optimization of sample treatment and reverse-phase separation conditions, tandem mass spectrometry detection was evaluated experimenting both positive and negative electrospray ionisation modes. The procedure was also preliminarily adapted to assess five arachidonic acid-derived eicosanoids that could be under the influence of vitamin E function, such as LTB4 (leukotriene B4), 20-HETE (20-hydroxyeicosatetraenoic acid) and their ω-oxidation metabolites. After the validation study, the performance characteristics were confirmed analysing a certified reference material (SRM 1950 - frozen human plasma by NIST). Finally, an application of the method in the analysis of lipid abnormalities of chronic kidney disease patients was shown. The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present, there are not procedures able to simultaneously determine these two classes of compounds. After the optimization of sample treatment and reverse-phase separation conditions, tandem mass spectrometry detection was evaluated experimenting both positive and negative electrospray ionisation modes. The procedure was also preliminarily adapted to assess five arachidonic acid-derived eicosanoids that could be under the influence of vitamin E function, such as LTB4 (leukotriene B4), 20-HETE (20-hydroxyeicosatetraenoic acid) and their ω-oxidation metabolites. After the validation study, the performance characteristics were confirmed analysing a certified reference material (SRM® 1950 - frozen human plasma by NIST). Finally, an application of the method in the analysis of lipid abnormalities of chronic kidney disease patients was shown.The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present, there are not procedures able to simultaneously determine these two classes of compounds. After the optimization of sample treatment and reverse-phase separation conditions, tandem mass spectrometry detection was evaluated experimenting both positive and negative electrospray ionisation modes. The procedure was also preliminarily adapted to assess five arachidonic acid-derived eicosanoids that could be under the influence of vitamin E function, such as LTB4 (leukotriene B4), 20-HETE (20-hydroxyeicosatetraenoic acid) and their ω-oxidation metabolites. After the validation study, the performance characteristics were confirmed analysing a certified reference material (SRM® 1950 - frozen human plasma by NIST). Finally, an application of the method in the analysis of lipid abnormalities of chronic kidney disease patients was shown.
Author	Bartolini, Desirée Gioiello, Antimo Giusepponi, Danilo Galarini, Roberta Torquato, Pierangelo Libetta, Carmelo Galli, Francesco Barola, Carolina Moretti, Simone Saluti, Giorgio
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Cites_doi	10.1002/rcm.1682 10.1089/bio.2015.0005 10.1016/j.freeradbiomed.2015.08.019 10.1016/j.freeradbiomed.2007.03.024 10.1046/j.1523-1755.2003.00151.x 10.1371/journal.pone.0201369 10.1016/S0261-5614(03)00128-6 10.1194/jlr.M009449 10.1016/j.freeradbiomed.2018.11.036 10.1016/j.jpba.2014.02.024 10.1016/j.freeradbiomed.2013.07.039 10.1007/s10238-014-0293-6 10.1016/j.cca.2013.11.008 10.1016/j.bbadis.2019.01.026 10.1016/j.freeradbiomed.2013.11.009 10.1016/j.talanta.2016.08.038 10.3390/antiox6040097 10.1016/j.freeradbiomed.2016.09.017 10.1021/acs.analchem.5b02794 10.1006/abio.2001.5486 10.1016/j.jchromb.2014.01.046 10.1016/j.plefa.2015.12.003 10.1016/j.clinbiochem.2013.08.008 10.1002/chir.20630 10.1016/j.talanta.2017.04.030 10.1016/j.jpba.2016.06.017 10.1016/j.jchromb.2013.06.014 10.1179/135100007X200353 10.1194/jlr.M079012 10.1080/01635580902825654 10.4155/bio.15.44 10.1016/j.freeradbiomed.2010.07.024 10.3390/antiox7010010 10.1007/s00216-012-6226-x
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Keywords	Validation Tocopheryl quinone Vitamin E Polyunsaturated fatty acids Eicosanoids Human plasma/serum Liquid-chromatography tandem-mass spectrometry (LC-MS/MS)
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References	Ciffolilli, Wallert, Bartolini, Krauth, Werz, Piroddi, Sebastiani, Torquato, Lorkowski, Birringer, Galli (bib12) 2015; 89 Metherel, Stark (bib33) 2016; 104 Simundic, Cornes, Grankvist, Lippi, Nybo (bib15) 2014; 432 Hinman, Holst, Latham, Bruegger, Ulas, McCusker, Amagata, Davis, Hoff, Kahn-Kirby, Kim, Kosaka, Lee, Malone, Mei, Richards, Rivera, Miller, Trimmer, Shrader (bib7) 2018; 13 Niki (bib3) 2007; 12 Wallert, Mosig, Rennert, Funke, Ristow, Pellegrino, Cruciani, Galli, Lorkowski, Birringer (bib11) 2014; 68 Aslan, Celmeli, Özcan, Kupesiz (bib34) 2015; 15 Galli, Birringer, Lorkowski, Frank, Azzi, Traber, Eggersdorfer, Ozer, Cook-Mills, Cruciani (bib4) 2017; 102 Traber, Atkinson J (bib1) 2007; 43 Le Faouder, Baillif, Spreadbury, Motta, Rousset, Chêne, Guigné, Tercé, Vanner, Vergnolle, Bertrand-Michel, Dubourdeau, Cenac (bib28) 2013; 932 Himmelfarb, Kane, McMonagle, Zaltas, Bobzin, Boddupalli, Phinney, Miller (bib35) 2003; 64 Quehenberger, Armando, Brown, Milne, Myers, Merrill, Bandyopadhyay, Jones, Kelly, Shaner, Sullards, Wang, Murphy, Barkley, Leiker, Raetz, Guan, Laird, Six, Russell, JD, McDonald, Subramaniam, Fahy, Dennis (bib37) 2010; 11 Giusepponi, Torquato, Bartolini, Piroddi, Birringer, Lorkowski, Libetta, Cruciani, Moretti, Saluti, Galli, Galarini (bib13) 2017; 170 Zheng, Ma, Han, Ye, Wang, Cai, Zhou, Yu, Zeng, Jiang (bib25) 2014; 95 Pein, Ville, Pace, Temml, Garscha, Raasch, Alsabil, Viault, Dinh, Guilet, Troisi, Neukirch, König, Bilancia, Waltenberger, Stuppner, Wallert, Lorkowski, Weinigel, Rummler, Birringer, Roviezzo, Sautebin, Helesbeux, Séraphin, Mosig, Schuster, Rossi, Richomme, Werz, Koeberle (bib5) 2018; 3834 Wolfer, Gaudin, Taylor-Robinson, Holmes, Kirk Nicholson (bib29) 2015; 87 Mottier, Gremaud, Guy, Turesky (bib20) 2002; 301 Ambrogini, Torquato, Bartolini, Albertini, Lattanzi, Palma, Marinelli, Betti, Minelli, Cuppini, Galli (bib2) 2019 Wang, Dai, Wang, Peng, Zhao, Bi (bib30) 2016; 161 Mohn, Kuchan, Erdman, Neuringer, Matthan, Chen, Johnson (bib8) 2017; 6 Strassburg, Huijbrechts, Kortekaas, Lindeman, Pedersen, Dane, Berger, Brenkman, Hankemeier, van Duynhoven, Kalkhoven, Newman, Vreeken (bib27) 2012; 404 Albahrani, Rotarou, Roche, Greaves (bib14) 2013; 46 Thakare, Chhonker, Gautam, Alamoudi, Alnouti (bib22) 2016; 128 Schubert, Kluge, Schmolz, Wallert, Galli, Birringer, Lorkowski (bib6) 2018; 7 Kortz, Dorowa, Ceglareka (bib24) 2014; 964 Zheng, Ma, Song, Ye, Wang, Tu, Li, Zhou, Zeng, Jiang (bib26) 2015; 79 Leonard, Bobe, Traber (bib40) 2019 Bowden, Heckert, Ulmer, Jones, Koelmel, Abdullah, Ahonen, Alnouti, Armando, Asara, Bamba, Barr, Bergquist, Borchers, Brandsma, Breitkopf, Cajka, Cazenave-Gassiot, Checa, Cinel, Colas, Cremers, Dennis, Evans, Fauland, Fiehn, Gardner, Garrett, Gotlinger, Han, Huang, Neo, Hyötyläinen, Izumi, Jiang, Jiang, Jiang, Kachman, Kiyonami, Klavins, Klose, Köfeler, Kolmert, Koal, Koster, Kuklenyik, Kurland, Leadley, Lin, Maddipati, McDougall, Meikle, Mellett, Monnin, Moseley, Nandakumar, Oresic, Patterson, Peake, Pierce, Post, Postle, Pugh, Qiu, Quehenberger, Ramrup, Rees, Rembiesa, Reynaud, Roth, Sales, Schuhmann, Schwartzman, Serhan, Shevchenko, Somerville, John-Williams, Surma, Takeda, Thakare, Thompson, Torta, Triebl, Trötzmüller, Kumari Ubhayasekera, Vuckovic, Weir, Welti, Wenk, Wheelock, Yao, Yuan, Zhao, Zhou (bib17) 2017; 58 Galli, Floridi, Floridi, Buoncristiani (bib36) 2004; 23 Torquato, Bartolini, Giusepponi, Piroddi, Sebastiani, Saluti, Galarini, Galli (bib21) 2019; 131 Albahrani, Greaves (bib16) 2016; 37 1 Montuschi, Martello, Felli, Mondino, Chiarotti (bib31) 2004; 18 Dorow, Becker, Kortz, Thiery, Hauschildt, Ceglarek (bib23) 2016; 14 Guidance For Industry (bib32) 2018 (bib38) 2015; 13 Lebold, Traber (bib9) 2014; 66 Campbel, Musich, Whaley, Stimmel, Leesnitzer, Dessus-Babus, Duffourc, Stone, Newman, Yang, Krishnan (bib10) 2009; 61 Torquato, Giusepponi, Galarini, Bartolini, Piroddi, Galli (bib39) 2019 Mazzini, Betti, Netscher, Galli, Salvadori (bib18) 2009; 21 Birringer, Lington, Vertuani, Manfredini, Scharlau, Glei, Ristow (bib19) 2010; 49 Campbel (10.1016/j.freeradbiomed.2019.04.017_bib10) 2009; 61 Himmelfarb (10.1016/j.freeradbiomed.2019.04.017_bib35) 2003; 64 Montuschi (10.1016/j.freeradbiomed.2019.04.017_bib31) 2004; 18 Strassburg (10.1016/j.freeradbiomed.2019.04.017_bib27) 2012; 404 (10.1016/j.freeradbiomed.2019.04.017_bib38) 2015; 13 Ambrogini (10.1016/j.freeradbiomed.2019.04.017_bib2) 2019 Schubert (10.1016/j.freeradbiomed.2019.04.017_bib6) 2018; 7 Mohn (10.1016/j.freeradbiomed.2019.04.017_bib8) 2017; 6 Hinman (10.1016/j.freeradbiomed.2019.04.017_bib7) 2018; 13 Torquato (10.1016/j.freeradbiomed.2019.04.017_bib21) 2019; 131 Leonard (10.1016/j.freeradbiomed.2019.04.017_bib40) 2019 Birringer (10.1016/j.freeradbiomed.2019.04.017_bib19) 2010; 49 Albahrani (10.1016/j.freeradbiomed.2019.04.017_bib16) 2016; 37 1 Quehenberger (10.1016/j.freeradbiomed.2019.04.017_bib37) 2010; 11 Mazzini (10.1016/j.freeradbiomed.2019.04.017_bib18) 2009; 21 Wang (10.1016/j.freeradbiomed.2019.04.017_bib30) 2016; 161 Zheng (10.1016/j.freeradbiomed.2019.04.017_bib25) 2014; 95 Kortz (10.1016/j.freeradbiomed.2019.04.017_bib24) 2014; 964 Ciffolilli (10.1016/j.freeradbiomed.2019.04.017_bib12) 2015; 89 Dorow (10.1016/j.freeradbiomed.2019.04.017_bib23) 2016; 14 Niki (10.1016/j.freeradbiomed.2019.04.017_bib3) 2007; 12 Zheng (10.1016/j.freeradbiomed.2019.04.017_bib26) 2015; 79 Galli (10.1016/j.freeradbiomed.2019.04.017_bib4) 2017; 102 Pein (10.1016/j.freeradbiomed.2019.04.017_bib5) 2018; 3834 Mottier (10.1016/j.freeradbiomed.2019.04.017_bib20) 2002; 301 Lebold (10.1016/j.freeradbiomed.2019.04.017_bib9) 2014; 66 Thakare (10.1016/j.freeradbiomed.2019.04.017_bib22) 2016; 128 Giusepponi (10.1016/j.freeradbiomed.2019.04.017_bib13) 2017; 170 Simundic (10.1016/j.freeradbiomed.2019.04.017_bib15) 2014; 432 Wolfer (10.1016/j.freeradbiomed.2019.04.017_bib29) 2015; 87 Torquato (10.1016/j.freeradbiomed.2019.04.017_bib39) 2019 Bowden (10.1016/j.freeradbiomed.2019.04.017_bib17) 2017; 58 Traber (10.1016/j.freeradbiomed.2019.04.017_bib1) 2007; 43 Wallert (10.1016/j.freeradbiomed.2019.04.017_bib11) 2014; 68 Galli (10.1016/j.freeradbiomed.2019.04.017_bib36) 2004; 23 Aslan (10.1016/j.freeradbiomed.2019.04.017_bib34) 2015; 15 Guidance For Industry (10.1016/j.freeradbiomed.2019.04.017_bib32) 2018 Albahrani (10.1016/j.freeradbiomed.2019.04.017_bib14) 2013; 46 Le Faouder (10.1016/j.freeradbiomed.2019.04.017_bib28) 2013; 932 Metherel (10.1016/j.freeradbiomed.2019.04.017_bib33) 2016; 104
References_xml	– volume: 15 start-page: 397 year: 2015 end-page: 403 ident: bib34 article-title: LC–MS/MS analysis of plasma polyunsaturated fatty acids in patients with homozygous sickle cell disease publication-title: Clin. Exp. Med. – volume: 43 start-page: 4 year: 2007 end-page: 15 ident: bib1 article-title: Vitamin E, antioxidant and nothing more publication-title: Free Radic. Biol. Med. – volume: 404 start-page: 1413 year: 2012 end-page: 1426 ident: bib27 article-title: Quantitative profiling of oxylipins through comprehensive LC-MS/MS analysis: application in cardiac surgery publication-title: Anal. Bioanal. Chem. – volume: 18 start-page: 2723 year: 2004 end-page: 2729 ident: bib31 article-title: Ion trap liquid chromatography/tandem mass spectrometry analysis of leukotriene B4 in exhaled breath condensate publication-title: Rapid Commun. Mass Spectrom. – volume: 49 start-page: 1315 year: 2010 end-page: 1322 ident: bib19 article-title: Proapoptotic effects of long-chain vitamin E metabolites in HepG2 cells are mediated by oxidative stress publication-title: Free Radic. Biol. Med. – year: 2019 ident: bib2 article-title: Excitotoxicity, neuroinflammation and oxidant stress as molecular bases of epileptogenesis and epilepsy-derived neurodegeneration: the role of vitamin E publication-title: Biochim. Biophys. Acta (BBA) - Mol. Basis Dis. – volume: 170 start-page: 552 year: 2017 end-page: 561 ident: bib13 article-title: Determination of tocopherols and their metabolites by liquid-chromatography coupled with tandem mass spectrometry in human plasma and serum publication-title: Talanta – volume: 102 start-page: 16 year: 2017 end-page: 36 ident: bib4 article-title: Vitamin E: emerging aspects and new directions publication-title: Free Radic. Biol. Med. – volume: 68 start-page: 43 year: 2014 end-page: 51 ident: bib11 article-title: Long-chain metabolites of alpha-tocopherol occur in human serum and inhibit macrophage foam cell formation in vitro publication-title: Free Radic. Biol. Med. – volume: 128 start-page: 426 year: 2016 end-page: 437 ident: bib22 article-title: Quantitative analysis of endogenous compound publication-title: J. Pharm. Biomed. Anal. – volume: 12 start-page: 204 year: 2007 end-page: 210 ident: bib3 article-title: Tocopherylquinone and tocopherylhydroquinone publication-title: Redox Rep. – volume: 13 start-page: 4149 year: 2015 ident: bib38 article-title: Scientific opinion on dietary reference values for vitamin E as α-tocopherol publication-title: EFSA J. – volume: 89 start-page: 952 year: 2015 end-page: 962 ident: bib12 article-title: Human serum determination and in vitro anti-inflammatory activity of the vitamin E metabolite alpha-(13'-hydroxy)-6-hydroxychroman publication-title: Free Radic. Biol. Med. – volume: 21 start-page: 519 year: 2009 end-page: 524 ident: bib18 article-title: Configuration of the vitamin E analogue garcinoic acid extracted from Garcinia Kola seeds publication-title: Chirality – volume: 6 start-page: 97 year: 2017 ident: bib8 article-title: The subcellular distribution of alpha-tocopherol in the adult primate brain and its relationship with membrane arachidonic acid and its oxidation products publication-title: Antioxidants – volume: 964 start-page: 1 year: 2014 end-page: 11 ident: bib24 article-title: Liquid chromatography–tandem mass spectrometry for the analysis of eicosanoids and related lipids in human biological matrices: a review publication-title: J. Chromatogr. B – volume: 932 start-page: 123 year: 2013 end-page: 133 ident: bib28 article-title: LC–MS/MS method for rapid and concomitant quantification of pro-inflammatory and pro-resolving polyunsaturated fatty acid metabolites publication-title: J. Chromatogr. B – volume: 11 start-page: 3299 year: 2010 end-page: 3305 ident: bib37 article-title: Lipidomics reveals a remarkable diversity of lipids in human plasma publication-title: J. Lipid Res. – volume: 95 start-page: 176 year: 2014 end-page: 183 ident: bib25 article-title: Post-column mobile phase adjustment: a strategy to eliminate the contradiction between liquid chromatography and mass spectrometry in the determination of flavonoids in rat plasma publication-title: J. Pharm. Biomed. Anal. – volume: 23 start-page: 205 year: 2004 end-page: 212 ident: bib36 article-title: Accumulation of vitamin E metabolites in the blood of renal failure patients publication-title: Clin. Nutr. – volume: 37 1 start-page: 27 year: 2016 end-page: 47 ident: bib16 article-title: Fat-soluble vitamins: clinical indications and current challenges for chromatographic measurement publication-title: Clin. Biochem. Rev. – volume: 301 start-page: 128 year: 2002 end-page: 135 ident: bib20 article-title: Comparison of gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry methods to quantify α-tocopherol and α-tocopherolquinone levels in human plasma publication-title: Anal. Biochem. – year: 2018 ident: bib32 article-title: Bioanalytical Method Validation – volume: 7 start-page: 10 year: 2018 ident: bib6 article-title: Long-chain metabolites of vitamin E: metabolic activation as a general concept for lipid-soluble vitamins? publication-title: Antioxidants – volume: 13 year: 2018 ident: bib7 article-title: Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase publication-title: PLoS One – volume: 432 start-page: 33 year: 2014 end-page: 37 ident: bib15 article-title: Standardization of collection requirements for fasting samples: for the working group on preanalytical phase (WG-PA) of the european federation of clinical chemistry and laboratory medicine (EFLM) publication-title: Clin. Chim. Acta – volume: 66 start-page: 13 year: 2014 end-page: 19 ident: bib9 article-title: Interactions between α-tocopherol, polyunsaturated fatty acids, and lipoxygenases during embryogenesis publication-title: Free Radic. Biol. Med. – volume: 131 start-page: 115 year: 2019 end-page: 125 ident: bib21 article-title: Increased plasma levels of the lipoperoxyl radical-derived vitamin E metabolite alpha-tocopheryl quinone are an early indicator of lipotoxicity in fatty liver subjects publication-title: Free Radic. Biol. Med. – volume: 161 start-page: 157 year: 2016 end-page: 164 ident: bib30 article-title: Simultaneous profiling of eicosanoid metabolome in plasma by UPLC–MS/MS method: application to identify potential makers for rheumatoid arthritis publication-title: Talanta – volume: 87 start-page: 11721 year: 2015 end-page: 11731 ident: bib29 article-title: Development and validation of a high throughput UHPLCMS approach for screening of oxylipins and their precursors publication-title: Anal. Chem. – volume: 79 start-page: 1081 year: 2015 end-page: 1891 ident: bib26 article-title: Simple and rapid determination of unsaturated fatty acids in 1 μl of rat plasma by LC–MS/MS publication-title: Bioanalysis – volume: 64 start-page: 978 year: 2003 end-page: 991 ident: bib35 article-title: Alpha and gamma tocopherol metabolism in healthy subjects and patients with end-stage renal disease publication-title: Kidney Int. – volume: 61 start-page: 649 year: 2009 end-page: 662 ident: bib10 article-title: Gamma tocopherol upregulates the expression of 15-S-HETE and induces growth arrest through a PPAR gamma-dependent mechanism in PC-3 human prostate cancer cells publication-title: Nutr. Canc. – volume: 14 start-page: 1 year: 2016 end-page: 7 ident: bib23 article-title: Preanalytical investigation of polyunsaturated fatty acids and eicosanoids in human plasma by liquid chromatography–tandem mass spectrometry publication-title: Biopreserv. Biobanking – start-page: 208 year: 2019 end-page: 227 ident: bib39 article-title: Analysis of vitamin E metabolites publication-title: Vitamin E: Chemistry and Nutritional Benefits – year: 2019 ident: bib40 article-title: Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months publication-title: J. Vitam. Nutr. Res. – volume: 58 start-page: 2275 year: 2017 end-page: 2288 ident: bib17 article-title: Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma publication-title: J. Lipid Res. – volume: 104 start-page: 33 year: 2016 end-page: 43 ident: bib33 article-title: The stability of blood fatty acids during storage and potential mechanisms of degradation: a review publication-title: Prostagl. Leukot. Essent. Fat. Acids – volume: 3834 start-page: 1 year: 2018 end-page: 17 ident: bib5 article-title: Endogenous metabolites of vitamin E limit inflammation by targeting 5-lipoxygenase publication-title: Nat. Commun. – volume: 46 start-page: 1884 year: 2013 end-page: 1888 ident: bib14 article-title: Comparison of three commercial calibrators for alpha-tocopherol using liquid chromatography-tandem mass spectrometry publication-title: Clin. Biochem. – volume: 18 start-page: 2723 year: 2004 ident: 10.1016/j.freeradbiomed.2019.04.017_bib31 article-title: Ion trap liquid chromatography/tandem mass spectrometry analysis of leukotriene B4 in exhaled breath condensate publication-title: Rapid Commun. Mass Spectrom. doi: 10.1002/rcm.1682 – volume: 14 start-page: 1 year: 2016 ident: 10.1016/j.freeradbiomed.2019.04.017_bib23 article-title: Preanalytical investigation of polyunsaturated fatty acids and eicosanoids in human plasma by liquid chromatography–tandem mass spectrometry publication-title: Biopreserv. Biobanking doi: 10.1089/bio.2015.0005 – volume: 89 start-page: 952 year: 2015 ident: 10.1016/j.freeradbiomed.2019.04.017_bib12 article-title: Human serum determination and in vitro anti-inflammatory activity of the vitamin E metabolite alpha-(13'-hydroxy)-6-hydroxychroman publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2015.08.019 – volume: 43 start-page: 4 issue: 1 year: 2007 ident: 10.1016/j.freeradbiomed.2019.04.017_bib1 article-title: Vitamin E, antioxidant and nothing more publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2007.03.024 – volume: 64 start-page: 978 year: 2003 ident: 10.1016/j.freeradbiomed.2019.04.017_bib35 article-title: Alpha and gamma tocopherol metabolism in healthy subjects and patients with end-stage renal disease publication-title: Kidney Int. doi: 10.1046/j.1523-1755.2003.00151.x – volume: 13 issue: 8 year: 2018 ident: 10.1016/j.freeradbiomed.2019.04.017_bib7 article-title: Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase publication-title: PLoS One doi: 10.1371/journal.pone.0201369 – volume: 23 start-page: 205 issue: 2 year: 2004 ident: 10.1016/j.freeradbiomed.2019.04.017_bib36 article-title: Accumulation of vitamin E metabolites in the blood of renal failure patients publication-title: Clin. Nutr. doi: 10.1016/S0261-5614(03)00128-6 – volume: 11 start-page: 3299 year: 2010 ident: 10.1016/j.freeradbiomed.2019.04.017_bib37 article-title: Lipidomics reveals a remarkable diversity of lipids in human plasma publication-title: J. Lipid Res. doi: 10.1194/jlr.M009449 – volume: 131 start-page: 115 year: 2019 ident: 10.1016/j.freeradbiomed.2019.04.017_bib21 article-title: Increased plasma levels of the lipoperoxyl radical-derived vitamin E metabolite alpha-tocopheryl quinone are an early indicator of lipotoxicity in fatty liver subjects publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2018.11.036 – volume: 95 start-page: 176 year: 2014 ident: 10.1016/j.freeradbiomed.2019.04.017_bib25 article-title: Post-column mobile phase adjustment: a strategy to eliminate the contradiction between liquid chromatography and mass spectrometry in the determination of flavonoids in rat plasma publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2014.02.024 – start-page: 208 year: 2019 ident: 10.1016/j.freeradbiomed.2019.04.017_bib39 article-title: Analysis of vitamin E metabolites – volume: 66 start-page: 13 year: 2014 ident: 10.1016/j.freeradbiomed.2019.04.017_bib9 article-title: Interactions between α-tocopherol, polyunsaturated fatty acids, and lipoxygenases during embryogenesis publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2013.07.039 – volume: 15 start-page: 397 year: 2015 ident: 10.1016/j.freeradbiomed.2019.04.017_bib34 article-title: LC–MS/MS analysis of plasma polyunsaturated fatty acids in patients with homozygous sickle cell disease publication-title: Clin. Exp. Med. doi: 10.1007/s10238-014-0293-6 – year: 2018 ident: 10.1016/j.freeradbiomed.2019.04.017_bib32 – volume: 432 start-page: 33 year: 2014 ident: 10.1016/j.freeradbiomed.2019.04.017_bib15 article-title: Standardization of collection requirements for fasting samples: for the working group on preanalytical phase (WG-PA) of the european federation of clinical chemistry and laboratory medicine (EFLM) publication-title: Clin. Chim. Acta doi: 10.1016/j.cca.2013.11.008 – volume: 37 1 start-page: 27 year: 2016 ident: 10.1016/j.freeradbiomed.2019.04.017_bib16 article-title: Fat-soluble vitamins: clinical indications and current challenges for chromatographic measurement publication-title: Clin. Biochem. Rev. – year: 2019 ident: 10.1016/j.freeradbiomed.2019.04.017_bib2 article-title: Excitotoxicity, neuroinflammation and oxidant stress as molecular bases of epileptogenesis and epilepsy-derived neurodegeneration: the role of vitamin E publication-title: Biochim. Biophys. Acta (BBA) - Mol. Basis Dis. doi: 10.1016/j.bbadis.2019.01.026 – volume: 68 start-page: 43 year: 2014 ident: 10.1016/j.freeradbiomed.2019.04.017_bib11 article-title: Long-chain metabolites of alpha-tocopherol occur in human serum and inhibit macrophage foam cell formation in vitro publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2013.11.009 – volume: 161 start-page: 157 year: 2016 ident: 10.1016/j.freeradbiomed.2019.04.017_bib30 article-title: Simultaneous profiling of eicosanoid metabolome in plasma by UPLC–MS/MS method: application to identify potential makers for rheumatoid arthritis publication-title: Talanta doi: 10.1016/j.talanta.2016.08.038 – volume: 6 start-page: 97 issue: 4 year: 2017 ident: 10.1016/j.freeradbiomed.2019.04.017_bib8 article-title: The subcellular distribution of alpha-tocopherol in the adult primate brain and its relationship with membrane arachidonic acid and its oxidation products publication-title: Antioxidants doi: 10.3390/antiox6040097 – volume: 102 start-page: 16 year: 2017 ident: 10.1016/j.freeradbiomed.2019.04.017_bib4 article-title: Vitamin E: emerging aspects and new directions publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2016.09.017 – volume: 87 start-page: 11721 issue: 23 year: 2015 ident: 10.1016/j.freeradbiomed.2019.04.017_bib29 article-title: Development and validation of a high throughput UHPLCMS approach for screening of oxylipins and their precursors publication-title: Anal. Chem. doi: 10.1021/acs.analchem.5b02794 – volume: 301 start-page: 128 year: 2002 ident: 10.1016/j.freeradbiomed.2019.04.017_bib20 article-title: Comparison of gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry methods to quantify α-tocopherol and α-tocopherolquinone levels in human plasma publication-title: Anal. Biochem. doi: 10.1006/abio.2001.5486 – year: 2019 ident: 10.1016/j.freeradbiomed.2019.04.017_bib40 article-title: Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months publication-title: J. Vitam. Nutr. Res. – volume: 964 start-page: 1 year: 2014 ident: 10.1016/j.freeradbiomed.2019.04.017_bib24 article-title: Liquid chromatography–tandem mass spectrometry for the analysis of eicosanoids and related lipids in human biological matrices: a review publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2014.01.046 – volume: 104 start-page: 33 year: 2016 ident: 10.1016/j.freeradbiomed.2019.04.017_bib33 article-title: The stability of blood fatty acids during storage and potential mechanisms of degradation: a review publication-title: Prostagl. Leukot. Essent. Fat. Acids doi: 10.1016/j.plefa.2015.12.003 – volume: 46 start-page: 1884 year: 2013 ident: 10.1016/j.freeradbiomed.2019.04.017_bib14 article-title: Comparison of three commercial calibrators for alpha-tocopherol using liquid chromatography-tandem mass spectrometry publication-title: Clin. Biochem. doi: 10.1016/j.clinbiochem.2013.08.008 – volume: 21 start-page: 519 issue: 5 year: 2009 ident: 10.1016/j.freeradbiomed.2019.04.017_bib18 article-title: Configuration of the vitamin E analogue garcinoic acid extracted from Garcinia Kola seeds publication-title: Chirality doi: 10.1002/chir.20630 – volume: 170 start-page: 552 year: 2017 ident: 10.1016/j.freeradbiomed.2019.04.017_bib13 article-title: Determination of tocopherols and their metabolites by liquid-chromatography coupled with tandem mass spectrometry in human plasma and serum publication-title: Talanta doi: 10.1016/j.talanta.2017.04.030 – volume: 128 start-page: 426 year: 2016 ident: 10.1016/j.freeradbiomed.2019.04.017_bib22 article-title: Quantitative analysis of endogenous compound publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2016.06.017 – volume: 932 start-page: 123 year: 2013 ident: 10.1016/j.freeradbiomed.2019.04.017_bib28 article-title: LC–MS/MS method for rapid and concomitant quantification of pro-inflammatory and pro-resolving polyunsaturated fatty acid metabolites publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2013.06.014 – volume: 13 start-page: 4149 issue: 7 year: 2015 ident: 10.1016/j.freeradbiomed.2019.04.017_bib38 article-title: Scientific opinion on dietary reference values for vitamin E as α-tocopherol publication-title: EFSA J. – volume: 12 start-page: 204 issue: 5 year: 2007 ident: 10.1016/j.freeradbiomed.2019.04.017_bib3 article-title: Tocopherylquinone and tocopherylhydroquinone publication-title: Redox Rep. doi: 10.1179/135100007X200353 – volume: 58 start-page: 2275 issue: 12 year: 2017 ident: 10.1016/j.freeradbiomed.2019.04.017_bib17 article-title: Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma publication-title: J. Lipid Res. doi: 10.1194/jlr.M079012 – volume: 61 start-page: 649 year: 2009 ident: 10.1016/j.freeradbiomed.2019.04.017_bib10 article-title: Gamma tocopherol upregulates the expression of 15-S-HETE and induces growth arrest through a PPAR gamma-dependent mechanism in PC-3 human prostate cancer cells publication-title: Nutr. Canc. doi: 10.1080/01635580902825654 – volume: 79 start-page: 1081 year: 2015 ident: 10.1016/j.freeradbiomed.2019.04.017_bib26 article-title: Simple and rapid determination of unsaturated fatty acids in 1 μl of rat plasma by LC–MS/MS publication-title: Bioanalysis doi: 10.4155/bio.15.44 – volume: 49 start-page: 1315 issue: 8 year: 2010 ident: 10.1016/j.freeradbiomed.2019.04.017_bib19 article-title: Proapoptotic effects of long-chain vitamin E metabolites in HepG2 cells are mediated by oxidative stress publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2010.07.024 – volume: 7 start-page: 10 issue: 1 year: 2018 ident: 10.1016/j.freeradbiomed.2019.04.017_bib6 article-title: Long-chain metabolites of vitamin E: metabolic activation as a general concept for lipid-soluble vitamins? publication-title: Antioxidants doi: 10.3390/antiox7010010 – volume: 404 start-page: 1413 year: 2012 ident: 10.1016/j.freeradbiomed.2019.04.017_bib27 article-title: Quantitative profiling of oxylipins through comprehensive LC-MS/MS analysis: application in cardiac surgery publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-012-6226-x – volume: 3834 start-page: 1 issue: 9 year: 2018 ident: 10.1016/j.freeradbiomed.2019.04.017_bib5 article-title: Endogenous metabolites of vitamin E limit inflammation by targeting 5-lipoxygenase publication-title: Nat. Commun.
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Snippet	The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study...
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SubjectTerms	Eicosanoids Human plasma/serum Liquid-chromatography tandem-mass spectrometry (LC-MS/MS) Polyunsaturated fatty acids Tocopheryl quinone Validation Vitamin E
Title	LC-MS/MS assay for the simultaneous determination of tocopherols, polyunsaturated fatty acids and their metabolites in human plasma and serum
URI	https://dx.doi.org/10.1016/j.freeradbiomed.2019.04.017 https://www.ncbi.nlm.nih.gov/pubmed/31009660 https://www.proquest.com/docview/2213155256
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