Elevating Limonene Production in Oleaginous Yeast Yarrowia lipolytica via Genetic Engineering of Limonene Biosynthesis Pathway and Optimization of Medium Composition
As an alternative terpenoid producer, non-conventional oleaginous yeast Yarrowia lipolytica was chosen for limonene production. Y. lipolytica can give high biomass yield and metabolize a broad range of substrates such as glycerol, alkanes, fatty acid, fats, and oils. As previously reported, optimiza...
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| Published in | Biotechnology and bioprocess engineering Vol. 24; no. 3; pp. 500 - 506 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Seoul
The Korean Society for Biotechnology and Bioengineering
01.06.2019
Springer Nature B.V 한국생물공학회 |
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| Abstract | As an alternative terpenoid producer, non-conventional oleaginous yeast
Yarrowia lipolytica
was chosen for limonene production.
Y. lipolytica
can give high biomass yield and metabolize a broad range of substrates such as glycerol, alkanes, fatty acid, fats, and oils. As previously reported, optimization of limonene synthesis pathway and mevalonate (MVA) pathway leads to the accumulation of 112-fold higher limonene as compared to an initial strain. In this study, we introduced an additional copy of limonene synthesis gene (
LS
), which resulted in an increase of limonene production. This engineered strain was used to carry out further optimization study. Amongst all the carbon sources tested, the highest level of limonene production was obtained from glycerol, and citrate was selected as an auxiliary carbon source. In fed-batch fermentation with an optimized medium, the engineered strain was found to produce 165.3 mg/L limonene, which corresponds to the highest yield till date for the production of limonene in
Y. lipolytica. |
|---|---|
| AbstractList | As an alternative terpenoid producer, non-conventional oleaginous yeast Yarrowia lipolytica was chosen for limonene production. Y. lipolytica can give high biomass yield and metabolize a broad range of substrates such as glycerol, alkanes, fatty acid, fats, and oils. As previously reported, optimization of limonene synthesis pathway and mevalonate (MVA) pathway leads to the accumulation of 112-fold higher limonene as compared to an initial strain. In this study, we introduced an additional copy of limonene synthesis gene (LS), which resulted in an increase of limonene production. This engineered strain was used to carry out further optimization study. Amongst all the carbon sources tested, the highest level of limonene production was obtained from glycerol, and citrate was selected as an auxiliary carbon source. In fed-batch fermentation with an optimized medium, the engineered strain was found to produce 165.3 mg/L limonene, which corresponds to the highest yield till date for the production of limonene in Y. lipolytica. As an alternative terpenoid producer, non-conventional oleaginous yeast Yarrowia lipolytica was chosen for limonene production. Y. lipolytica can give high biomass yield and metabolize a broad range of substrates such as glycerol, alkanes, fatty acid, fats, and oils. As previously reported, optimization of limonene synthesis pathway and mevalonate (MVA) pathway leads to the accumulation of 112-fold higher limonene as compared to an initial strain. In this study, we introduced an additional copy of limonene synthesis gene ( LS ), which resulted in an increase of limonene production. This engineered strain was used to carry out further optimization study. Amongst all the carbon sources tested, the highest level of limonene production was obtained from glycerol, and citrate was selected as an auxiliary carbon source. In fed-batch fermentation with an optimized medium, the engineered strain was found to produce 165.3 mg/L limonene, which corresponds to the highest yield till date for the production of limonene in Y. lipolytica. As an alternative terpenoid producer, nonconventional oleaginous yeast Yarrowia lipolytica was chosen for limonene production. Y. lipolytica can give high biomass yield and metabolize a broad range of substrates such as glycerol, alkanes, fatty acid, fats, and oils. As previously reported, optimization of limonene synthesis pathway and mevalonate (MVA) pathway leads to the accumulation of 112-fold higher limonene as compared to an initial strain. In this study, we introduced an additional copy of limonene synthesis gene (LS), which resulted in an increase of limonene production. This engineered strain was used to carry out further optimization study. Amongst all the carbon sources tested, the highest level of limonene production was obtained from glycerol, and citrate was selected as an auxiliary carbon source. In fed-batch fermentation with an optimized medium, the engineered strain was found to produce 165.3 mg/L limonene, which corresponds to the highest yield till date for the production of limonene in Y. lipolytica. KCI Citation Count: 0 |
| Author | Wei, Liu-Jing Chen, Jun Lv, Yu-Bei Cheng, Bo-Qian Hua, Qiang |
| Author_xml | – sequence: 1 givenname: Bo-Qian surname: Cheng fullname: Cheng, Bo-Qian organization: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology – sequence: 2 givenname: Liu-Jing surname: Wei fullname: Wei, Liu-Jing email: weiliujing@ecust.edu.cn organization: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology – sequence: 3 givenname: Yu-Bei surname: Lv fullname: Lv, Yu-Bei organization: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology – sequence: 4 givenname: Jun surname: Chen fullname: Chen, Jun organization: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology – sequence: 5 givenname: Qiang surname: Hua fullname: Hua, Qiang organization: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT) |
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| Snippet | As an alternative terpenoid producer, non-conventional oleaginous yeast
Yarrowia lipolytica
was chosen for limonene production.
Y. lipolytica
can give high... As an alternative terpenoid producer, non-conventional oleaginous yeast Yarrowia lipolytica was chosen for limonene production. Y. lipolytica can give high... As an alternative terpenoid producer, nonconventional oleaginous yeast Yarrowia lipolytica was chosen for limonene production. Y. lipolytica can give high... |
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| SubjectTerms | Alkanes Batch culture batch fermentation biomass production Biosynthesis Biotechnology Carbon Carbon sources Chemistry Chemistry and Materials Science citrates Citric acid Fats fatty acids Fermentation genes Genetic engineering Glycerol Industrial and Production Engineering Limonene Mevalonate pathway Mevalonic acid oils Oils & fats Optimization Research Paper Substrates Yarrowia lipolytica Yeast yeasts 생물공학 |
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| Title | Elevating Limonene Production in Oleaginous Yeast Yarrowia lipolytica via Genetic Engineering of Limonene Biosynthesis Pathway and Optimization of Medium Composition |
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