Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans

• Published in: BioTechniques Vol. 43; no. 5; pp. 596, 598, 560 - 600
• Main Authors: LYSSENKO, Nicholas N, HANNA-ROSE, Wendy, SCHLEGEL, Robert A
• Format: Journal Article
• Language: English
• Published: Natick, MA Eaton 01.11.2007; Taylor & Francis Group
• Subjects: Animals; Biological and medical sciences; Caenorhabditis elegans - genetics; Cell Nucleus - metabolism; Diverse techniques; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes, Reporter; Green Fluorescent Proteins - metabolism; Molecular and cellular biology; Nuclear Localization Signals - metabolism; Protein Transport; Caenorhabditis elegans; Signal; Reporter gene; Helmintha; Nemathelminthia; Invertebrata; Gene expression; Localization; Nematoda

Targeting a gene expression reporter, usually the green fluorescent protein (GFP), to the nucleus via a translationally fused nuclear localization signal (NLS) greatly facilitates recognition and identification of the reporter-expressing cells in Caenorhabditis elegans. Presently circulating nematod...