Contents of eicosapentaenoic acid and docosahexaenoic acid in "Health food" [contained eicosapentaenoic acid, Japan]

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 26; no. 5; pp. 500 - 506_1
• Main Authors: Maeda, Y. (Shizuoka-ken. Inst. of Public Health and Environmental Science (Japan)), Ishikawa, M, Yamamoto, M, Terada, S, Masui, T, Watanabe, Y
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society for Food Hygiene and Safety 01.01.1985
• Subjects: ACEITES DE PESCADO; acid value; ACIDE GRAS; ACIDOS GRASOS; ALIMENT DE REGIME; ALIMENTOS DIETETICOS; ANALISIS QUIMICO; ANALYSE CHIMIQUE; CHEMICAL ANALYSIS; CHROMATOGRAPHIE EN PHASE GAZEUSE; CI-mass chromatogram; CROMATOGRAFIA DE GASES; DIETETIC FOODS; docosahexaenoic acid (DHA); eicosapentaenoic acid (EPA); ENFERMEDADES VASCULARES; fatty acid composition; FATTY ACIDS; FISH OILS; GAS CHROMATOGRAPHY; gas liquid chromatography (FID); HEALTH; health food; HUILE DE POISSON; JAPAN; JAPON; MALADIE VASCULAIRE; peroxide value; SALUD; SANTE; THROMBOSE; THROMBOSIS; tocopherol; triphenyl ethylene; TROMBOSIS; VASCULAR DISEASES
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.26.500

A simple method for the determination of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in “health food” contained EPA by gas liquid chromatography (GC) was developed. A sample of 100mg was methyl-esterified by the boron trifluoride-methanol (BF3-MeOH) method and the product was extracted with hexane containing triphenyl ethylene as an internal standard. The sample solution was determined by GC on a 10% Silar 10C column. Recoveries of EPA and DHA added to oils were in the range of 98.8-107% and 98.8-122%, respectively. The present method was applied to “health food” contained EPA. The results showed thet the quantities of EPA and DHA (mg) per gram of oils and fats of the supplements were 20.3 and 11.4 on average, respectively. Moreover, the fatty acid composition, acid value (AV), peroxide value (POV) and tocopherols (Toc) in the “health food” were investigated. Though the “health food” is easily taken, from the viewpoint of safety, nutritional balance and price, EPA and DHA should be taken in the diet in the form of sardines, mackerels, and so on.