Assessing the responses of cellular proteins induced by hyaluronic acid-modified surfaces utilizing a mass spectrometry-based profiling system: Over-expression of CD36, CD44, CDK9, and PP2A

• Published in: Analyst (London) Vol. 137; no. 21; pp. 4921 - 4933
• Main Authors: Yang, Ming-Hui, Jong, Shiang-Bin, Lu, Chi-Yu, Lin, Yu-Fen, Chiang, Pei-Wen, Tyan, Yu-Chang, Chung, Tze-Wen
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 07.11.2012
• Subjects: Amino Acid Sequence; Analytical chemistry; Biological and medical sciences; Bromodeoxyuridine - metabolism; CD36 Antigens - chemistry; CD36 Antigens - metabolism; Cell Line; Cell Proliferation - drug effects; Chemistry; Chitosan - chemistry; Cyclin-Dependent Kinase 9 - chemistry; Cyclin-Dependent Kinase 9 - metabolism; Diverse techniques; Electrodes; Exact sciences and technology; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; General, instrumentation; Humans; Hyaluronan Receptors - chemistry; Hyaluronan Receptors - metabolism; Hyaluronic Acid - chemistry; Hyaluronic Acid - pharmacology; Mass Spectrometry; Molecular and cellular biology; Molecular Sequence Data; Nanotubes, Carbon - chemistry; Protein Phosphatase 2 - chemistry; Protein Phosphatase 2 - metabolism; Proteomics - methods; Spectrometric and optical methods; Surface Properties; Cell proliferation; Antibody; Use; Carbon nanotubes; Adhesion; Density; Survival; Protein; Chemical modification; Cover; Morphological analysis; Biopolymer; Proteomics; Response surface; Chitosan; Technique; Surface area; Differential method; Fibroblast; Mass spectrometry; Flow injection; Quartz microbalance

The cell responses to biopolymer surface at the early adhesion stages can be critical for cell survival. The purpose of this research was to assess formation of hyaluronic acid (HA) biopolymer surface, the fibroblasts were used as an experimental model to evaluate the responses of cellular proteins...